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Cloning and expansion of repetitive DNA sequences

Williams, Sophie L ; Coster, Gideon

Methods in cell biology, 2024, Vol.182, p.167-185 [Periódico revisado por pares]

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Título:
Cloning and expansion of repetitive DNA sequences
Autor: Williams, Sophie L ; Coster, Gideon
Assuntos: Base Sequence ; Cloning, Molecular ; DNA - genetics ; Humans ; Repetitive Sequences, Nucleic Acid - genetics
É parte de: Methods in cell biology, 2024, Vol.182, p.167-185
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Repeat and structure-prone DNA sequences comprise a large proportion of the human genome. The instability of these sequences has been implicated in a range of diseases, including cancers and neurodegenerative disorders. However, the mechanism of pathogenicity is poorly understood. As such, further studies on repetitive DNA are required. Cloning and maintaining repeat-containing substrates is challenging due to their inherent ability to form non-B DNA secondary structures which are refractory to DNA polymerases and prone to undergo rearrangements. Here, we describe an approach to clone and expand tandem-repeat DNA without interruptions, thereby allowing for its manipulation and subsequent investigation.
Editor: United States
Idioma: Inglês

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