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Genome-scale metabolic analysis of Clostridium thermocellum for bioethanol production
Roberts, Seth B ; Gowen, Christopher M ; Brooks, J. Paul ; Fong, Stephen S
VCU Scholars Compass 2010
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Título:
Genome-scale metabolic analysis of Clostridium thermocellum for bioethanol production
Autor:
Roberts, Seth B
;
Gowen, Christopher M
;
Brooks, J. Paul
;
Fong, Stephen S
Assuntos:
Engineering
;
Physical Sciences and Mathematics
Notas:
https://scholarscompass.vcu.edu/context/clse_pubs/article/1000/filename/1/type/additional/viewcontent/1752_0509_4_31_s2.txt
https://scholarscompass.vcu.edu/context/clse_pubs/article/1000/filename/2/type/additional/viewcontent/1752_0509_4_31_s3.xml
info:doi/10.1186/1752-0509-4-31
https://scholarscompass.vcu.edu/context/clse_pubs/article/1000/filename/4/type/additional/viewcontent/1752_0509_4_31_s5.doc
https://scholarscompass.vcu.edu/context/clse_pubs/article/1000/filename/0/type/additional/viewcontent/1752_0509_4_31_s1.xls
https://scholarscompass.vcu.edu/context/clse_pubs/article/1000/filename/3/type/additional/viewcontent/1752_0509_4_31_s4.txt
https://scholarscompass.vcu.edu/context/clse_pubs/article/1000/viewcontent/1752_0509_4_31.pdf
https://scholarscompass.vcu.edu/clse_pubs/1
Chemical and Life Science Engineering Publications
Descrição:
Background Microorganisms possess diverse metabolic capabilities that can potentially be leveraged for efficient production of biofuels. Clostridium thermocellum (ATCC 27405) is a thermophilic anaerobe that is both cellulolytic and ethanologenic, meaning that it can directly use the plant sugar, cellulose, and biochemically convert it to ethanol. A major challenge in using microorganisms for chemical production is the need to modify the organism to increase production efficiency. The process of properly engineering an organism is typically arduous. Results Here we present a genome-scale model of C. thermocellum metabolism, iSR432, for the purpose of establishing a computational tool to study the metabolic network of C. thermocellum and facilitate efforts to engineer C. thermocellum for biofuel production. The model consists of 577 reactions involving 525 intracellular metabolites, 432 genes, and a proteomic-based representation of a cellulosome. The process of constructing this metabolic model led to suggested annotation refinements for 27 genes and identification of areas of metabolism requiring further study. The accuracy of the iSR432 model was tested using experimental growth and by-product secretion data for growth on cellobiose and fructose. Analysis using this model captures the relationship between the reduction-oxidation state of the cell and ethanol secretion and allowed for prediction of gene deletions and environmental conditions that would increase ethanol production. Conclusions By incorporating genomic sequence data, network topology, and experimental measurements of enzyme activities and metabolite fluxes, we have generated a model that is reasonably accurate at predicting the cellular phenotype of C. thermocellum and establish a strong foundation for rational strain design. In addition, we are able to draw some important conclusions regarding the underlying metabolic mechanisms for observed behaviors of C. thermocellum and highlight remaining gaps in the existing genome annotations.
Editor:
VCU Scholars Compass
Data de criação/publicação:
2010
Idioma:
Inglês
Links
View record in Virginia Commonwealth University$$FView record in $$GVirginia Commonwealth University
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