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A 3D Searchable Database of Transgenic Zebrafish Gal4 and Cre Lines for Functional Neuroanatomy Studies

Marquart, Gregory D ; Tabor, Kathryn M ; Brown, Mary ; Strykowski, Jennifer L ; Varshney, Gaurav K ; LaFave, Matthew C ; Mueller, Thomas ; Burgess, Shawn M ; Higashijima, Shin-Ichi ; Burgess, Harold A

Frontiers in neural circuits, 2015-11, Vol.9, p.78-78 [Periódico revisado por pares]

Switzerland: Frontiers Research Foundation

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Título:
A 3D Searchable Database of Transgenic Zebrafish Gal4 and Cre Lines for Functional Neuroanatomy Studies
Autor: Marquart, Gregory D ; Tabor, Kathryn M ; Brown, Mary ; Strykowski, Jennifer L ; Varshney, Gaurav K ; LaFave, Matthew C ; Mueller, Thomas ; Burgess, Shawn M ; Higashijima, Shin-Ichi ; Burgess, Harold A
Assuntos: 3' Untranslated regions ; 3D registration ; Animals ; Animals, Genetically Modified - anatomy & histology ; Animals, Genetically Modified - growth & development ; Animals, Genetically Modified - metabolism ; Artificial chromosomes ; Brain - cytology ; Brain - metabolism ; Brain architecture ; Brain research ; Cardiac muscle ; Circuits ; Confocal microscopy ; cre ; Databases, Factual ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Fertilization ; Functional anatomy ; Functional morphology ; Gal4 ; Gene Expression ; Gene mapping ; Genes ; Genetic engineering ; Genomes ; Genomics ; Imaging, Three-Dimensional - methods ; Insects ; microRNA ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Microscopy, Confocal - methods ; miRNA ; Morphology ; Muscles - cytology ; Muscles - metabolism ; Myocardium - cytology ; Myocardium - metabolism ; Nervous system ; Neural networks ; Neurons ; Neuroscience ; Neurosciences ; Questioning ; Registration ; Science ; Skin ; Skin - cytology ; Skin - metabolism ; Software ; Transcription Factors - genetics ; Transcription Factors - metabolism ; transgenic ; Visualization ; Zebrafish ; Zebrafish - anatomy & histology ; Zebrafish - growth & development ; Zebrafish - metabolism ; Zebrafish Proteins - genetics ; Zebrafish Proteins - metabolism
É parte de: Frontiers in neural circuits, 2015-11, Vol.9, p.78-78
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Edited by: Claire Wyart, Brain and Spinal Cord Institute (ICM), France
Reviewed by: Filippo Del Bene, Institut Curie, France; Vatsala Thirumalai, National Centre for Biological Sciences, India; Isaac Henry Bianco, University College London, UK
Descrição: Transgenic methods enable the selective manipulation of neurons for functional mapping of neuronal circuits. Using confocal microscopy, we have imaged the cellular-level expression of 109 transgenic lines in live 6 day post fertilization larvae, including 80 Gal4 enhancer trap lines, 9 Cre enhancer trap lines and 20 transgenic lines that express fluorescent proteins in defined gene-specific patterns. Image stacks were acquired at single micron resolution, together with a broadly expressed neural marker, which we used to align enhancer trap reporter patterns into a common 3-dimensional reference space. To facilitate use of this resource, we have written software that enables searching for transgenic lines that label cells within a selectable 3-dimensional region of interest (ROI) or neuroanatomical area. This software also enables the intersectional expression of transgenes to be predicted, a feature which we validated by detecting cells with co-expression of Cre and Gal4. Many of the imaged enhancer trap lines show intrinsic brain-specific expression. However, to increase the utility of lines that also drive expression in non-neuronal tissue we have designed a novel UAS reporter, that suppresses expression in heart, muscle, and skin through the incorporation of microRNA binding sites in a synthetic 3' untranslated region. Finally, we mapped the site of transgene integration, thus providing molecular identification of the expression pattern for most lines. Cumulatively, this library of enhancer trap lines provides genetic access to 70% of the larval brain and is therefore a powerful and broadly accessible tool for the dissection of neural circuits in larval zebrafish.
Editor: Switzerland: Frontiers Research Foundation
Idioma: Inglês

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A 3D Searchable Database of Transgenic Zebrafish Gal4 and Cre Lines for Functional Neuroanatomy Studies

Marquart, Gregory D ; Tabor, Kathryn M ; Brown, Mary ; Strykowski, Jennifer L ; Varshney, Gaurav K ; LaFave, Matthew C ; Mueller, Thomas ; Burgess, Shawn M ; Higashijima, Shin-Ichi ; Burgess, Harold A

Switzerland: Frontiers Research Foundation

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