Idioma:

Bcl2L12 Contributes to Th2-Biased Inflammation in the Intestinal Mucosa by Regulating CD4 + T Cell Activities

Li, Mao-Gang ; Liu, Xiao-Yu ; Liu, Zhi-Qiang ; Hong, Jing-Yi ; Liu, Jiang-Qi ; Zhou, Cai-Jie ; Hu, Tian-Yong ; Xiao, Xiao-Jun ; Ran, Pi-Xin ; Zheng, Peng-Yuan ; Liu, Zhi-Gang ; Yang, Ping-Chang

The Journal of immunology (1950), 2018-07, Vol.201 (2), p.725-733 [Periódico revisado por pares]

United States: American Association of Immunologists

Texto completo disponível

Citações Citado por

Enviar para

Título:
Bcl2L12 Contributes to Th2-Biased Inflammation in the Intestinal Mucosa by Regulating CD4 + T Cell Activities
Autor: Li, Mao-Gang ; Liu, Xiao-Yu ; Liu, Zhi-Qiang ; Hong, Jing-Yi ; Liu, Jiang-Qi ; Zhou, Cai-Jie ; Hu, Tian-Yong ; Xiao, Xiao-Jun ; Ran, Pi-Xin ; Zheng, Peng-Yuan ; Liu, Zhi-Gang ; Yang, Ping-Chang
Assuntos: Adult ; Animals ; Apoptosis ; CD4 antigen ; Cells, Cultured ; Colon ; Cytokines ; Cytokines - metabolism ; Deregulation ; Female ; Flow cytometry ; GATA-3 protein ; Helper cells ; Humans ; Inflammation ; Inflammation - immunology ; Inflammatory bowel diseases ; Inflammatory Bowel Diseases - immunology ; Interleukin 4 ; Intestinal Mucosa - immunology ; Intestine ; Lymphocyte Activation ; Lymphocytes ; Lymphocytes T ; Male ; Mice ; Mice, Inbred BALB C ; Mucosa ; Muscle Proteins - metabolism ; Pathogenesis ; Peripheral blood ; Polymerase chain reaction ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; RNA, Small Interfering - genetics ; Th2 Cells - immunology ; Therapeutic applications ; Ulcerative colitis ; Western blotting ; Young Adult
É parte de: The Journal of immunology (1950), 2018-07, Vol.201 (2), p.725-733
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: The Th2-biased inflammation and immune deregulation play a critical role in the pathogenesis of ulcerative colitis (UC). Recent studies indicate that the Bcl2-like protein 12 (Bcl2L12) is associated with immune deregulation of UC. This study aims to investigate the role of Bcl2L12 in the induction of aberrant Th2-biased inflammation. In this study, peripheral blood samples were collected from patients with inflammatory bowel disease. The Th2 cell activities were analyzed by flow cytometry, real-time quantitative RT-PCR, and Western blotting. Mice with Bcl2L12-knockout CD4 T cells were used in the experiments. The results showed that the expression of Bcl2L12 was detected in peripheral CD4 T cells, which was significantly higher in UC patients than in healthy subjects. A positive correlation between the expression of Bcl2L12 and Th2 cytokines was detected in CD4 T cells from UC patients. Naive CD4 T cells with Bcl2L12 overexpression were prone to differentiate into Th2 cells. Mice with Bcl2L12 deficiency failed to induce the Th2-biased inflammation in the intestine. Bcl2L12 bound GATA3 to form a complex to enhance the binding between GATA3 and the promoter to enhance the expression of IL-4 in CD4 T cells. CD4 T cells with Bcl2L12 overexpression were resistant to apoptosis. In conclusion, the Bcl2L12 is a critical factor in the induction of aberrant Th2 polarization by upregulating Th2 responses and downregulating Th2 cell apoptosis. Bcl2L12 may be a novel therapeutic target in the management of the disorders with Th2-biased inflammation.
Editor: United States: American Association of Immunologists
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Bcl2L12 Contributes to Th2-Biased Inflammation in the Intestinal Mucosa by Regulating CD4 + T Cell Activities

Li, Mao-Gang ; Liu, Xiao-Yu ; Liu, Zhi-Qiang ; Hong, Jing-Yi ; Liu, Jiang-Qi ; Zhou, Cai-Jie ; Hu, Tian-Yong ; Xiao, Xiao-Jun ; Ran, Pi-Xin ; Zheng, Peng-Yuan ; Liu, Zhi-Gang ; Yang, Ping-Chang

United States: American Association of Immunologists

Buscando em bases de dados remotas. Favor aguardar.