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Silencing circSLAMF6 represses cell glycolysis, migration, and invasion by regulating the miR-204-5p/MYH9 axis in gastric cancer under hypoxia

Fang, Xinhui ; Bai, Yangqiu ; Zhang, Lida ; Ding, Songze

Bioscience reports, 2020-06, Vol.40 (6) [Periódico revisado por pares]

England: Portland Press Ltd

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Título:
Silencing circSLAMF6 represses cell glycolysis, migration, and invasion by regulating the miR-204-5p/MYH9 axis in gastric cancer under hypoxia
Autor: Fang, Xinhui ; Bai, Yangqiu ; Zhang, Lida ; Ding, Songze
Assuntos: Animals ; Cancer ; Cell Line, Tumor ; Cell Movement ; Gene Expression Regulation, Neoplastic ; Gene Silencing ; Glycolysis ; Humans ; Male ; Mice, Nude ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Myosin Heavy Chains - genetics ; Myosin Heavy Chains - metabolism ; Neoplasm Invasiveness ; RNA, Circular - genetics ; RNA, Circular - metabolism ; Signal Transduction ; Stomach Neoplasms - genetics ; Stomach Neoplasms - metabolism ; Stomach Neoplasms - pathology ; Tumor Hypoxia ; Tumor Microenvironment
É parte de: Bioscience reports, 2020-06, Vol.40 (6)
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Gastric cancer (GC) is a malignant tumor of the digestive tract. Hypoxia plays an important role in the development of cancer, including GC. The present study aimed to investigate the role of circular RNA SLAMF6 (circSLAMF6) in the progression of GC under hypoxia. The expression of circSLAMF6, microRNA-204-5p (miR-204-5p) and myosin heavy chain 9 (MYH9) was measured by quantitative real-time polymerase chain reaction (qRT-PCR). GC cells were maintained under hypoxia (1% O2) for experiments in vitro. Glucose consumption and lactate production were determined by a Glucose Assay Kit and a Lactate Assay Kit, respectively. Levels of all protein were detected by Western blot. Cell migration and invasion were examined by Transwell assay. The interaction between miR-204-5p and circSLAMF6 or MYH9 was analyzed by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Murine xenograft model was established to explore the role of circSLAMF6 in vivo. CircSLAMF6 expression was increased in GC cells under hypoxia. Hypoxia promoted glycolysis, migration, and invasion in GC cells, which were reversed by circSLAMF6 knockdown. CircSLAMF6 was validated as a miR-204-5p sponge, and MYH9 was a target of miR-204-5p. Functionally, miR-204-5p inhibitor weakened the inhibition of circSLAMF6 knockdown on GC cell progression under hypoxia. Besides, MYH9 depletion suppressed glycolysis, migration, and invasion in GC cells under hypoxia. Importantly, circSLAMF6 deficiency inhibited tumor growth in vivo by regulating the miR-204-5p/MYH9 axis. CircSLAMF6 was involved in glycolysis, migration, and invasion by regulating the miR-204-5p/MYH9 axis in GC cells under hypoxia.
Editor: England: Portland Press Ltd
Idioma: Inglês

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Silencing circSLAMF6 represses cell glycolysis, migration, and invasion by regulating the miR-204-5p/MYH9 axis in gastric cancer under hypoxia

Fang, Xinhui ; Bai, Yangqiu ; Zhang, Lida ; Ding, Songze

England: Portland Press Ltd

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