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Cloning of L-lactate dehydrogenase and elimination of lactic acid production via gene knockout in Thermoanaerobacterium saccharolyticum JW/SL-YS485

Desai, S.G ; Guerinot, M.L ; Lynd, L.R

Applied microbiology and biotechnology, 2004-10, Vol.65 (5), p.600-605 [Revista revisada por pares]

Berlin: Springer

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Título:
Cloning of L-lactate dehydrogenase and elimination of lactic acid production via gene knockout in Thermoanaerobacterium saccharolyticum JW/SL-YS485
Autor: Desai, S.G ; Guerinot, M.L ; Lynd, L.R
Materias: Acetic Acid - analysis ; Acids ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - physiology ; Biological and medical sciences ; Biological Sciences ; Biotechnology ; Cell Proliferation ; Cloning, Molecular ; Dehydrogenases ; DNA, Bacterial - chemistry ; DNA, Bacterial - isolation & purification ; Ethanol - analysis ; Fundamental and applied biological sciences. Psychology ; Gene Deletion ; Genes, Bacterial ; Glucose ; Glucose - metabolism ; L-Lactate Dehydrogenase - genetics ; L-Lactate Dehydrogenase - physiology ; Lactic Acid - metabolism ; metabolic engineering ; Microbiology ; Molecular Sequence Data ; Recombination, Genetic ; Sequence Analysis, DNA ; Thermoanaerobacterium - enzymology ; Thermoanaerobacterium - growth & development ; Thermoanaerobacterium saccharolyticum ; Xylose - metabolism
Es parte de: Applied microbiology and biotechnology, 2004-10, Vol.65 (5), p.600-605
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descripción: The gene encoding L-lactate dehydrogenase from Thermoanaerobacterium saccharolyticum JW/SL-YS485 was cloned, sequenced, and used to obtain an L-ldh deletion mutant strain (TD1) following a site-specific double-crossover event as confirmed by PCR and Southern blot. Growth rates and final cell densities were similar for strain TD1 and the wild-type grown on glucose and xylose. Lactic acid was below the limit of detection (0.3 mM) for strain TD1 on both glucose and xylose at all times tested, but was readily detected for the wild-type strain, with average final concentrations of 8.1 and 1.8 mM on glucose and xylose, respectively. Elimination of lactic acid as a fermentation product was accompanied by a proportional increase in the yields of acetic acid and ethanol. The results reported here represent a step toward using metabolic engineering to develop strains of thermophilic anaerobic bacteria that do not produce organic acids, and support the methodological feasibility of this goal.
Editor: Berlin: Springer
Idioma: Inglés

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Cloning of L-lactate dehydrogenase and elimination of lactic acid production via gene knockout in Thermoanaerobacterium saccharolyticum JW/SL-YS485

Desai, S.G ; Guerinot, M.L ; Lynd, L.R

Berlin: Springer

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