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Vesicle-independent extracellular release of a proinflammatory outer membrane lipoprotein in free-soluble form

Karched, Maribasappa ; Ihalin, Riikka ; Eneslätt, Kjell ; Zhong, Deyu ; Oscarsson, Jan ; Wai, Sun N ; Chen, Casey ; Asikainen, Sirkka E

BMC microbiology, 2008-01, Vol.8 (1), p.18-18, Article 18 [Periódico revisado por pares]

England: BioMed Central Ltd

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Título:
Vesicle-independent extracellular release of a proinflammatory outer membrane lipoprotein in free-soluble form
Autor: Karched, Maribasappa ; Ihalin, Riikka ; Eneslätt, Kjell ; Zhong, Deyu ; Oscarsson, Jan ; Wai, Sun N ; Chen, Casey ; Asikainen, Sirkka E
Assuntos: Actinomycetales ; Aggregatibacter actinomycetemcomitans - pathogenicity ; Aggregatibacter actinomycetemcomitans - physiology ; Aggregatibacter actinomycetemcomitans - ultrastructure ; Bacterial Outer Membrane Proteins - metabolism ; Blood lipoproteins ; Causes of ; Cell Culture Techniques ; Cell Membrane - ultrastructure ; Genetic aspects ; Health aspects ; Humans ; Lipoproteins ; Lipoproteins - immunology ; Lipoproteins - metabolism ; ODONTOLOGI ; ODONTOLOGY ; Periodontitis ; Proteolipids
É parte de: BMC microbiology, 2008-01, Vol.8 (1), p.18-18, Article 18
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Aggregatibacter actinomycetemcomitans is an oral bacterium associated with aggressively progressing periodontitis. Extracellular release of bacterial outer membrane proteins has been suggested to mainly occur via outer membrane vesicles. This study investigated the presence and conservation of peptidoglycan-associated lipoprotein (AaPAL) among A. actinomycetemcomitans strains, the immunostimulatory effect of AaPAL, and whether live cells release this structural outer membrane lipoprotein in free-soluble form independent of vesicles. The pal locus and its gene product were confirmed in clinical A. actinomycetemcomitans strains by PCR-restriction fragment length polymorphism and immunoblotting. Culturing under different growth conditions revealed no apparent requirement for the AaPAL expression. Inactivation of pal in a wild-type strain (D7S) and in its spontaneous laboratory variant (D7SS) resulted in pleiotropic cellular effects. In a cell culture insert model (filter pore size 0.02 mum), AaPAL was detected from filtrates when strains D7S and D7SS were incubated in serum or broth in the inserts. Electron microscopy showed that A. actinomycetemcomitans vesicles (0.05-0.2 mum) were larger than the filter pores and that there were no vesicles in the filtrates. The filtrates were immunoblot negative for a cytoplasmic marker, cyclic AMP (cAMP) receptor protein. An ex vivo model indicated cytokine production from human whole blood stimulated by AaPAL. Free-soluble AaPAL can be extracellularly released in a process independent of vesicles.
Editor: England: BioMed Central Ltd
Idioma: Inglês;Sueco

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Vesicle-independent extracellular release of a proinflammatory outer membrane lipoprotein in free-soluble form

Karched, Maribasappa ; Ihalin, Riikka ; Eneslätt, Kjell ; Zhong, Deyu ; Oscarsson, Jan ; Wai, Sun N ; Chen, Casey ; Asikainen, Sirkka E

England: BioMed Central Ltd

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