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Whole-Brain Imaging with Single-Cell Resolution Using Chemical Cocktails and Computational Analysis

Susaki, Etsuo A. ; Tainaka, Kazuki ; Perrin, Dimitri ; Kishino, Fumiaki ; Tawara, Takehiro ; Watanabe, Tomonobu M. ; Yokoyama, Chihiro ; Onoe, Hirotaka ; Eguchi, Megumi ; Yamaguchi, Shun ; Abe, Takaya ; Kiyonari, Hiroshi ; Shimizu, Yoshihiro ; Miyawaki, Atsushi ; Yokota, Hideo ; Ueda, Hiroki R.

Cell, 2014-04, Vol.157 (3), p.726-739 [Periódico revisado por pares]

United States: Elsevier Inc

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  • Título:
    Whole-Brain Imaging with Single-Cell Resolution Using Chemical Cocktails and Computational Analysis
  • Autor: Susaki, Etsuo A. ; Tainaka, Kazuki ; Perrin, Dimitri ; Kishino, Fumiaki ; Tawara, Takehiro ; Watanabe, Tomonobu M. ; Yokoyama, Chihiro ; Onoe, Hirotaka ; Eguchi, Megumi ; Yamaguchi, Shun ; Abe, Takaya ; Kiyonari, Hiroshi ; Shimizu, Yoshihiro ; Miyawaki, Atsushi ; Yokota, Hideo ; Ueda, Hiroki R.
  • Assuntos: Animals ; Brain - cytology ; Callithrix ; Indicators and Reagents - chemistry ; Mice ; Microscopy - methods ; Neuroimaging - methods
  • É parte de: Cell, 2014-04, Vol.157 (3), p.726-739
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
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  • Descrição: Systems-level identification and analysis of cellular circuits in the brain will require the development of whole-brain imaging with single-cell resolution. To this end, we performed comprehensive chemical screening to develop a whole-brain clearing and imaging method, termed CUBIC (clear, unobstructed brain imaging cocktails and computational analysis). CUBIC is a simple and efficient method involving the immersion of brain samples in chemical mixtures containing aminoalcohols, which enables rapid whole-brain imaging with single-photon excitation microscopy. CUBIC is applicable to multicolor imaging of fluorescent proteins or immunostained samples in adult brains and is scalable from a primate brain to subcellular structures. We also developed a whole-brain cell-nuclear counterstaining protocol and a computational image analysis pipeline that, together with CUBIC reagents, enable the visualization and quantification of neural activities induced by environmental stimulation. CUBIC enables time-course expression profiling of whole adult brains with single-cell resolution. [Display omitted] •Simple and efficient brain-clearing reagents and image informatics were developed•CUBIC is applicable to imaging of fluorescent proteins in adult brains and 3D-IHC•The CUBIC method is scalable from a primate brain to subcellular structures•CUBIC enables whole-brain imaging with single-cell resolution CUBIC enables rapid whole-brain imaging with single-photon excitation microscopy and scaling from subcellular structures to a primate brain.
  • Editor: United States: Elsevier Inc
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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