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Complement is necessary for full human monocyte differentiation into dendritic cells

E S Reis José Alexandre Marzagão Barbuto; Lourdes Isaac; Meeting of the Brazilian Society for Immunology (31. 2006 Búzios)

Abstracts São Paulo, SP: Brazilian Society for Immunology, 2006

São Paulo 2006

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  • Título:
    Complement is necessary for full human monocyte differentiation into dendritic cells
  • Autor: E S Reis
  • José Alexandre Marzagão Barbuto; Lourdes Isaac; Meeting of the Brazilian Society for Immunology (31. 2006 Búzios)
  • Assuntos: IMUNOLOGIA
  • É parte de: Abstracts São Paulo, SP: Brazilian Society for Immunology, 2006
  • Notas: Disponível em CD-ROM
  • Descrição: Introduction and Objectives: Dendritic cells (DCs) and complement are important components of innate immunity; both participate in maintenance of tolerance and link innate and acquired immunity. Considering that DCs are present at most tissues and inflammatory sites and that they are a source of several complement proteins, we investigated a possible role for complement in the differentiation of human monocytes into DCs. Methods and Results: DCs were generated in vitro from leukocytes obtained by leukapheresis from healthy donors. Adherent peripheral blood mononuclear cells were isolated and cultured in the presence of GMCSF plus IL-4 in culture medium supplemented with normal human serum (DC-SHN) or C3 deficient serum (DC-C3D). DCs obtained by day 7 were CD14-CD1ahigh, CD11c+HLA-DR+ and had typical DC morphology. For the activation of DCs, LPS was added in the last 24 h of culture, resulting in an increase in the expression of co-stimulatory molecules. When compared to DCs-C3D, DCs-SHN presented a higher expression of CD1a [MFI: 43 (DCs-SHN) x 29 (DCs-C3D)], HLA-DR (MFI: 179 x 122), CD209 (MFI: 315 x 202) and of the co-stimulatory molecules, CD80 (MFI: 105 x 67) and CD86 (MFI: 135 x 85) as observed by flow cytometry. Furthermore, DCs-SHN also secreted higher levels of IL-6 [mean concentration: 110 (DCs-SHN) x 58 pg/ml (DCs-C3D)] (detected by ELISA), but similar levels of IL-10, IL-12p40 and IL-12p70. The expression of CD11c, CD205,
    CD83, CR1 and CD18 was not significantly different between the 2 groups. LPS stimulation of DCs induced lower expression of CD1a (MFI: 26 x 36), HLA-DR (MFI: 166 x 99) and CD209 (MFI: 184 x 121) in DCs-C3D, which also produced less IL-12p70 (23 x 100 pg/ml) than DCs cultivated in the presence of C3 (DCs-SHN). Conclusion: Our results suggest a participation of complement in DC differentiation. We are now investigating if complement- dependent processes contribute to DC activation of T cells and in the modulation of the immune response.
  • Editor: São Paulo
  • Data de criação/publicação: 2006
  • Formato: res. IN.026.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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