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Optimization of the enzymatic hydrolysis of Moringa oleifera Lam oil using molecular docking analysis for fatty acid specificity

Barbosa, Milson S. ; Freire, Cintia C. C. ; Almeida, Lays C. ; Freitas, Lisiane S. ; Souza, Ranyere L. ; Pereira, Ernandes B. ; Mendes, Adriano A. ; Pereira, Matheus M. ; Lima, Álvaro S. ; Soares, Cleide M. F.

Biotechnology and applied biochemistry, 2019-09, Vol.66 (5), p.823-832 [Periódico revisado por pares]

United States: Wiley Subscription Services, Inc

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Título:
Optimization of the enzymatic hydrolysis of Moringa oleifera Lam oil using molecular docking analysis for fatty acid specificity
Autor: Barbosa, Milson S. ; Freire, Cintia C. C. ; Almeida, Lays C. ; Freitas, Lisiane S. ; Souza, Ranyere L. ; Pereira, Ernandes B. ; Mendes, Adriano A. ; Pereira, Matheus M. ; Lima, Álvaro S. ; Soares, Cleide M. F.
Assuntos: Acid production ; Biocatalysis ; Biocatalysts ; Biofuels ; Buffer solutions ; Burkholderia ; Catalysis ; commercial lipases ; Design optimization ; Emulsifiers ; enzymatic hydrolysis ; Fatty acids ; Fatty Acids - analysis ; Free energy ; Hydrolysis ; Lipase ; Lipase - metabolism ; Molecular docking ; Molecular Docking Simulation ; Moringa oleifera ; Moringa oleifera - chemistry ; Moringa oleifera - metabolism ; Moringa oleifera Lam oil ; Oil ; Oleic acid ; Pancreas ; Plant Oils - chemistry ; Plant Oils - metabolism ; Pseudomonas fluorescens ; Response surface methodology ; surface response methodology ; Sustainability ; Temperature effects
É parte de: Biotechnology and applied biochemistry, 2019-09, Vol.66 (5), p.823-832
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Alternative strategies are required to develop the optimized production of fatty acids using biocatalysis; molecular docking and response surface methodology are efficient tools to achieve this goal. In the present study, we demonstrate a novel and robust methodology for the sustainable production of fatty acids from Moringa oleifera Lam oil using lipase‐catalyzed hydrolysis (without the presence of emulsifiers or buffer solutions). Seven commercial lipases from Candida rugosa (CRL), Burkholderia cepacia (BCL), Thermomyces lanuginosus (TLL), Rhizopus niveus (RNL), Pseudomonas fluorescens (PFL), Mucor javanicus (MJL), and porcine pancreas (PPL) were used as biocatalysts. Initial screening showed that CRL had the highest hydrolytic activity (hydrolysis degree of 81%). Molecular docking analysis contributed to the experimental results, showing that CRL displays more stable binding free energy with oleic acid (C18:1), which is the fatty acid of highest concentration in Moringa oleifera Lam oil. To evaluate and optimize the hydrolysis process, response surface methodology (RSM) was used. The effect of temperature, mass ratio oil:water, and hydrolytic activity on enzymatic hydrolysis was evaluated by central composite design using RSM. Under the optimized conditions (temperature of 37 °C, mass ratio oil:water of 25%, and hydrolytic activity of 550 U goil−1), the maximum hydrolysis degree (100%) was achieved. The present study provides a robust method for the enzymatic hydrolysis of different oils for efficient and sustainable fatty acid production.
Editor: United States: Wiley Subscription Services, Inc
Idioma: Inglês

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Optimization of the enzymatic hydrolysis of Moringa oleifera Lam oil using molecular docking analysis for fatty acid specificity

Barbosa, Milson S. ; Freire, Cintia C. C. ; Almeida, Lays C. ; Freitas, Lisiane S. ; Souza, Ranyere L. ; Pereira, Ernandes B. ; Mendes, Adriano A. ; Pereira, Matheus M. ; Lima, Álvaro S. ; Soares, Cleide M. F.

United States: Wiley Subscription Services, Inc

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