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Effect of water storage and heat treatment on the cytotoxicity of soft liners

Tay, Lidia Yileng ; Herrera, Daniel Rodrigo ; Quishida, Cristiane Campos Costa ; Carlos, Iracilda Zeppone ; Jorge, Janaina Habib

Gerodontology, 2012-06, Vol.29 (2), p.e275-e280 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

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Título:
Effect of water storage and heat treatment on the cytotoxicity of soft liners
Autor: Tay, Lidia Yileng ; Herrera, Daniel Rodrigo ; Quishida, Cristiane Campos Costa ; Carlos, Iracilda Zeppone ; Jorge, Janaina Habib
Assuntos: acrylic resin ; Acrylic Resins - chemistry ; Acrylic Resins - toxicity ; Animals ; biocompatibility ; Cell Line ; Cell Survival - drug effects ; cell viability ; Culture Media, Conditioned ; Dental Materials - chemistry ; Dental Materials - toxicity ; Denture Liners ; Dimethylpolysiloxanes - chemistry ; Dimethylpolysiloxanes - toxicity ; Fibroblasts - drug effects ; Hot Temperature ; Materials Testing ; Methylmethacrylates - chemistry ; Methylmethacrylates - toxicity ; Mice ; polymethyl methacrylate ; Radiopharmaceuticals ; Silicone Elastomers - chemistry ; Silicone Elastomers - toxicity ; Temperature ; Thymidine ; Time Factors ; Tritium ; Water
É parte de: Gerodontology, 2012-06, Vol.29 (2), p.e275-e280
Notas: istex:D6660AFA0A5209D533421D06CFA649276CA83B3C
ark:/67375/WNG-C5QFZ4MR-Z
ArticleID:GER463
Descrição: doi: 10.1111/j.1741‐2358.2011.00463.x Effect of water storage and heat treatment on the cytotoxicity of soft liners Objective: To evaluate the effect of water storage time on the cytotoxicity of soft liners. Methods: Sample discs of soft liners Dentusoft, Dentuflex, Trusoft, Ufi‐Gel‐P and denture base acrylic resin Lucitone‐550 were prepared and divided into four groups: GN: No treatment, G24: Stored in water at 37°C for 24 h; G48: Stored in water at 37°C for 48 h, GHW: Immersed in water at 55°C for 10 min. To analyse the cytotoxic effect, three samples of each group were placed in tubes with Dubelcco’s Modified Eagle Mediums and incubated at 37°C for 24 h. During this period, the toxic substances were leached to the culture medium. The cytotoxicity was analysed quantitatively by the incorporation of radioactivity 3H‐thymidine checking the number of viable cells (synthesis of DNA). The data were statistically analysed using two‐way anova and Tukey’s honestly significant difference tests (α = 0.05). Results: Treatments did not reduce the cytotoxicity effect of the soft liners (p > 0.05). It was found that Ufi‐Gel‐P had a non‐cytotoxic effect, Trusoft had a slightly cytotoxic effect, Dentuflex had a moderated cytotoxic effect, Dentusoft alternated between slightly and non‐cytotoxic effect, and Lucitone‐550 had non‐cytotoxic effect when stored in water for 48 h. Conclusion: The effect of water storage and the heat treatment did not reduce the cytotoxicity of the soft liners.
Editor: Oxford, UK: Blackwell Publishing Ltd
Idioma: Inglês

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Effect of water storage and heat treatment on the cytotoxicity of soft liners

Tay, Lidia Yileng ; Herrera, Daniel Rodrigo ; Quishida, Cristiane Campos Costa ; Carlos, Iracilda Zeppone ; Jorge, Janaina Habib

Oxford, UK: Blackwell Publishing Ltd

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