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Positive and negative elements involved in the differential regulation by heme and oxygen of the HEM13 gene (coproporphyrinogen oxidase) in Saccharomyces cerevisiae

Amillet, J.M ; Buisson, N ; Labbe-Bois, R. (Universite Paris 7. Paris (France). Lab. de Biochimie des Porphyrines. Inst. Jacques Monod)

Current genetics, 1995-11, Vol.28 (6), p.503-511 [Periódico revisado por pares]

United States

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Título:
Positive and negative elements involved in the differential regulation by heme and oxygen of the HEM13 gene (coproporphyrinogen oxidase) in Saccharomyces cerevisiae
Autor: Amillet, J.M ; Buisson, N ; Labbe-Bois, R. (Universite Paris 7. Paris (France). Lab. de Biochimie des Porphyrines. Inst. Jacques Monod)
Assuntos: Base Sequence ; beta-Galactosidase - biosynthesis ; beta-Galactosidase - genetics ; Coproporphyrinogen Oxidase - genetics ; Coproporphyrinogen Oxidase - metabolism ; DNA-Binding Proteins - genetics ; Fungal Proteins - genetics ; GENE ; Gene Deletion ; Gene Expression Regulation, Fungal ; GENES ; Heme - genetics ; Molecular Sequence Data ; Nuclear Proteins ; OXIGENO ; Oxygen ; OXYGENE ; PORFIRINAS ; PORPHYRINE ; Recombinant Fusion Proteins - biosynthesis ; Recombinant Fusion Proteins - genetics ; Repressor Proteins - genetics ; SACCHAROMYCES CEREVISIAE ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins ; Trans-Activators - genetics ; Transcription Factors ; Transcription, Genetic
É parte de: Current genetics, 1995-11, Vol.28 (6), p.503-511
Notas: F30
97B6671
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: The Saccharomyces cerevisiae HEM13 gene codes for coproporphyrinogen oxidase (CPO), an oxygen-requiring enzyme catalysing the sixth step of heme biosynthesis. Its transcription is increased 40-5O-fold in response to oxygen- or heme-deficiency. CPO activity and HEM13 mRNA levels was analysed in a set of isogenic strains carrying single or double deletions of the CYP1 (HAP1), ROX1, SSN6, or TUP1 genes. The cells were grown in the presence or absence of oxygen and under heme-deficiency (hem1 Delta background). Both Rox1p and Cyp1p partially repressed HEM13 in aerobic heme-sufficient cells, probably in an independent manner. In the absence of heme, Cyp1p activated HEM13 and strongly repressed ROX1, allowing de-repression of HEM13. Cyp1p had no effect on HEM13 expression in anaerobic cells. Deletions of SSN6 or TUP1 dramatically de-repressed HEM13 in aerobic cells. A series of deletions in the HEM13 promoter identified at least four regulatory regions that are required for HEM13 regulation. Two regions, containing motifs similar to the Rox1p consensus sequences, act as repression sites under aerobic growth. The two other sites act as activation sequences required for full induction under oxygen- or heme-deficiency. Taken together, these results suggest that induction of HEM13 occurs in part through relief of repression exerted by Rox1p and Cyp1p, and in part by activation mediated partly by Cyp1p under heme-deficiency and by unknown factors under oxygen-deficiency.
Editor: United States
Idioma: Inglês

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Positive and negative elements involved in the differential regulation by heme and oxygen of the HEM13 gene (coproporphyrinogen oxidase) in Saccharomyces cerevisiae

Amillet, J.M ; Buisson, N ; Labbe-Bois, R. (Universite Paris 7. Paris (France). Lab. de Biochimie des Porphyrines. Inst. Jacques Monod)

United States

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