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Triggering of Cultured Neoplastic Mast Cells by Antibodies to the Receptor for IgE

Isersky, Chaviva ; Taurog, Joel D ; Poy, George ; Metzger, Henry

The Journal of immunology (1950), 1978-08, Vol.121 (2), p.549-558 [Periódico revisado por pares]

United States: Am Assoc Immnol

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Título:
Triggering of Cultured Neoplastic Mast Cells by Antibodies to the Receptor for IgE
Autor: Isersky, Chaviva ; Taurog, Joel D ; Poy, George ; Metzger, Henry
Assuntos: Animals ; Antibodies, Anti-Idiotypic ; Antibodies, Neoplasm ; Binding Sites, Antibody ; Binding, Competitive ; Cell Membrane - metabolism ; Cells, Cultured ; Dose-Response Relationship, Immunologic ; Electrophoresis, Polyacrylamide Gel ; Immunoglobulin E ; Iodine Radioisotopes ; Mast Cells - immunology ; Mice ; Neoplasms, Experimental - immunology ; Rabbits ; Rats
É parte de: The Journal of immunology (1950), 1978-08, Vol.121 (2), p.549-558
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Cell surface receptors for IgE were isolated from detergent lysates of iodinated, IgE-saturated, rat basophilic leukemia cells by precipitation with anti-IgE antibodies followed by chromatography at acid pH. The isolated material showed a single 125I-band (m.w. approximately 58,000) on gel electrophoresis in sodium dodecyl sulfate and was used to immunize a rabbit. The resulting anti-serum was reacted with lysates of surface iodinated mouse or rat tumor mast cells. Analysis of the precipitates on (10%) gel electrophoresis revealed one major peak comprising greater than 80% of the detectable counts and having an estimated m.w. of approximately 58,000. The antiserum reacted with detergent-solubilized and cell-bound receptors in the presence or absence of excess IgE; it also inhibited the binding of 125I-IgE. Cultured mouse mastocytoma cells never exposed to IgE released 3H-serotonin when incubated with F(ab')2, but not Fab' fragments of the antiserum, which had been rigorously freed of IgE and anti-IgE. The release was inhibited in the presence of excess IgE, was Ca++ dependent, and equaled 80% of the maximum obtained with IgE and anti-IgE. We conclude that aggregation of the receptors for IgE provides the critical signals for cell activation.
Editor: United States: Am Assoc Immnol
Idioma: Inglês

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Triggering of Cultured Neoplastic Mast Cells by Antibodies to the Receptor for IgE

Isersky, Chaviva ; Taurog, Joel D ; Poy, George ; Metzger, Henry

United States: Am Assoc Immnol

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