Idioma:

Iron Chaperone Poly rC Binding Protein 1 Protects Mouse Liver From Lipid Peroxidation and Steatosis

Protchenko, Olga ; Baratz, Ethan ; Jadhav, Shyamalagauri ; Li, Fengmin ; Shakoury‐Elizeh, Minoo ; Gavrilova, Oksana ; Ghosh, Manik C. ; Cox, James E. ; Maschek, J. Alan ; Tyurin, Vladimir A. ; Tyurina, Yulia Y. ; Bayir, Hülya ; Aron, Allegra T. ; Chang, Christopher J. ; Kagan, Valerian E. ; Philpott, Caroline C.

Hepatology (Baltimore, Md.), 2021-03, Vol.73 (3), p.1176-1193 [Periódico revisado por pares]

United States: Wiley Subscription Services, Inc

Texto completo disponível

Citações Citado por

Enviar para

Título:
Iron Chaperone Poly rC Binding Protein 1 Protects Mouse Liver From Lipid Peroxidation and Steatosis
Autor: Protchenko, Olga ; Baratz, Ethan ; Jadhav, Shyamalagauri ; Li, Fengmin ; Shakoury‐Elizeh, Minoo ; Gavrilova, Oksana ; Ghosh, Manik C. ; Cox, James E. ; Maschek, J. Alan ; Tyurin, Vladimir A. ; Tyurina, Yulia Y. ; Bayir, Hülya ; Aron, Allegra T. ; Chang, Christopher J. ; Kagan, Valerian E. ; Philpott, Caroline C.
Assuntos: Animals ; Antioxidants ; Cell death ; Degeneration ; Dietary supplements ; DNA-Binding Proteins - metabolism ; Fatty liver ; Fatty Liver - etiology ; Fatty Liver - metabolism ; Fatty Liver - pathology ; Female ; Ferroptosis ; Gene expression ; Glutathione peroxidase ; Hepatocytes ; Hepatocytes - metabolism ; Hepatology ; Homeostasis ; Humans ; Inflammation ; Iron ; Iron Compounds - metabolism ; Iron deficiency ; Lipid Peroxidation ; Lipids ; Liver - metabolism ; Liver - pathology ; Liver diseases ; Male ; Mammalian cells ; Metallochaperones - metabolism ; Mice ; Mice, Knockout ; Oxidants ; Oxidative Stress ; Proteins ; Reactive oxygen species ; RNA-Binding Proteins - metabolism ; Steatosis ; Toxicity ; Transcriptomes ; Vitamin E
É parte de: Hepatology (Baltimore, Md.), 2021-03, Vol.73 (3), p.1176-1193
Notas: Author Contributions: O.P., S.J., and E.B. conceived and designed the studies, performed experiments, analyzed data, discussed results, and wrote the manuscript. F.L. and M.S.-E. designed studies, performed experiments, and analyzed data. O.G., H.S., M.G., J.E.C., J.A.M., V.A.T., Y.Y.T., and H.B. performed experiments and analyzed data. A.T.A. and C.J.C. provided reagents and discussion and helped analyze imaging results. V.E.K. designed studies, analyzed data, and discussed results. C.C.P. conceived and designed studies, analyzed data, discussed results, and wrote the manuscript.
Descrição: Background and Aims Iron is essential yet also highly chemically reactive and potentially toxic. The mechanisms that allow cells to use iron safely are not clear; defects in iron management are a causative factor in the cell‐death pathway known as ferroptosis. Poly rC binding protein 1 (PCBP1) is a multifunctional protein that serves as a cytosolic iron chaperone, binding and transferring iron to recipient proteins in mammalian cells. Although PCBP1 distributes iron in cells, its role in managing iron in mammalian tissues remains open for study. The liver is highly specialized for iron uptake, utilization, storage, and secretion. Approach and Results Mice lacking PCBP1 in hepatocytes exhibited defects in liver iron homeostasis with low levels of liver iron, reduced activity of iron enzymes, and misregulation of the cell‐autonomous iron regulatory system. These mice spontaneously developed liver disease with hepatic steatosis, inflammation, and degeneration. Transcriptome analysis indicated activation of lipid biosynthetic and oxidative‐stress response pathways, including the antiferroptotic mediator, glutathione peroxidase type 4. Although PCBP1‐deleted livers were iron deficient, dietary iron supplementation did not prevent steatosis; instead, dietary iron restriction and antioxidant therapy with vitamin E prevented liver disease. PCBP1‐deleted hepatocytes exhibited increased labile iron and production of reactive oxygen species (ROS), were hypersensitive to iron and pro‐oxidants, and accumulated oxidatively damaged lipids because of the reactivity of unchaperoned iron. Conclusions Unchaperoned iron in PCBP1‐deleted mouse hepatocytes leads to production of ROS, resulting in lipid peroxidation (LPO) and steatosis in the absence of iron overload. The iron chaperone activity of PCBP1 is therefore critical for limiting the toxicity of cytosolic iron and may be a key factor in preventing the LPO that triggers the ferroptotic cell‐death pathway.
Editor: United States: Wiley Subscription Services, Inc
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 3 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Iron Chaperone Poly rC Binding Protein 1 Protects Mouse Liver From Lipid Peroxidation and Steatosis

United States: Wiley Subscription Services, Inc

Buscando em bases de dados remotas. Favor aguardar.