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Automated Tools to Advance High-Resolution Imaging in Liquid

Jonaid, G. M. ; Casasanta, Michael A. ; Dearnaley, William J. ; Berry, Samantha ; Kaylor, Liam ; Dressel-Dukes, Madeline J. ; Spilman, Michael S. ; Gray, Jennifer L. ; Kelly, Deborah F.

Microscopy and microanalysis, 2022-04, Vol.28 (2), p.361-370 [Periódico revisado por pares]

New York, USA: Cambridge University Press

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Título:
Automated Tools to Advance High-Resolution Imaging in Liquid
Autor: Jonaid, G. M. ; Casasanta, Michael A. ; Dearnaley, William J. ; Berry, Samantha ; Kaylor, Liam ; Dressel-Dukes, Madeline J. ; Spilman, Michael S. ; Gray, Jennifer L. ; Kelly, Deborah F.
Assuntos: Automation ; Biological activity ; Biological computing ; COVID-19 ; Data collection ; Electron microscopy ; Graphene ; High resolution ; Image resolution ; Macromolecules ; Medical research ; Microscopy ; N protein ; Nucleocapsids ; Oligomerization ; Particle size ; Proteins ; Room temperature ; Semiconductors ; Severe acute respiratory syndrome coronavirus 2 ; Silicon nitride ; Software ; Software and Instrumentation ; Specimen preparation ; Symmetry ; Vaccine development ; Vaccines ; Viral diseases ; Viruses
É parte de: Microscopy and microanalysis, 2022-04, Vol.28 (2), p.361-370
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Descrição: Liquid-electron microscopy (EM), the room-temperature correlate to cryo-EM, is a rapidly growing field providing high-resolution insights of macromolecules in solution. Here, we describe how liquid-EM experiments can incorporate automated tools to propel the field to new heights. We demonstrate fresh workflows for specimen preparation, data collection, and computing processes to assess biological structures in liquid. Adeno-associated virus (AAV) and the SARS-CoV-2 nucleocapsid (N) were used as model systems to highlight the technical advances. These complexes were selected based on their major differences in size and natural symmetry. AAV is a highly symmetric, icosahedral assembly with a particle diameter of ~25 nm. At the other end of the spectrum, N protein is an asymmetric monomer or dimer with dimensions of approximately 5–7 nm, depending upon its oligomerization state. Equally important, both AAV and N protein are popular subjects in biomedical research due to their high value in vaccine development and therapeutic efforts against COVID-19. Overall, we demonstrate how automated practices in liquid-EM can be used to decode molecules of interest for human health and disease.
Editor: New York, USA: Cambridge University Press
Idioma: Inglês

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Automated Tools to Advance High-Resolution Imaging in Liquid

Jonaid, G. M. ; Casasanta, Michael A. ; Dearnaley, William J. ; Berry, Samantha ; Kaylor, Liam ; Dressel-Dukes, Madeline J. ; Spilman, Michael S. ; Gray, Jennifer L. ; Kelly, Deborah F.

New York, USA: Cambridge University Press

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