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The catalytic activity of the translation termination factor methyltransferase Mtq2-Trm112 complex is required for large ribosomal subunit biogenesis

Lacoux, Caroline ; Wacheul, Ludivine ; Saraf, Kritika ; Pythoud, Nicolas ; Huvelle, Emmeline ; Figaro, Sabine ; Graille, Marc ; Carapito, Christine ; Lafontaine, Denis L J ; Heurgué-Hamard, Valérie

Nucleic acids research, 2020-12, Vol.48 (21), p.12310-12325 [Periódico revisado por pares]

England: Oxford University Press

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Título:
The catalytic activity of the translation termination factor methyltransferase Mtq2-Trm112 complex is required for large ribosomal subunit biogenesis
Autor: Lacoux, Caroline ; Wacheul, Ludivine ; Saraf, Kritika ; Pythoud, Nicolas ; Huvelle, Emmeline ; Figaro, Sabine ; Graille, Marc ; Carapito, Christine ; Lafontaine, Denis L J ; Heurgué-Hamard, Valérie
Assuntos: Binding Sites ; Biocatalysis ; Biochemistry ; Biochemistry, Molecular Biology ; Cellular Biology ; Cloning, Molecular ; Crystallography, X-Ray ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Gene Expression Regulation, Fungal ; Genetic Vectors - chemistry ; Genetic Vectors - metabolism ; Life Sciences ; Methyltransferases - chemistry ; Methyltransferases - genetics ; Methyltransferases - metabolism ; Models, Molecular ; Organelle Biogenesis ; Peptide Chain Termination, Translational ; Peptide Termination Factors - genetics ; Peptide Termination Factors - metabolism ; Protein Binding ; Protein Conformation, alpha-Helical ; Protein Conformation, beta-Strand ; Protein Interaction Domains and Motifs ; Recombinant Proteins - chemistry ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Ribosome Subunits, Large, Eukaryotic - genetics ; Ribosome Subunits, Large, Eukaryotic - metabolism ; RNA and RNA-protein complexes ; RNA, Ribosomal - biosynthesis ; RNA, Ribosomal - genetics ; RNA, Ribosomal, 5.8S - biosynthesis ; RNA, Ribosomal, 5.8S - genetics ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - chemistry ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Substrate Specificity ; tRNA Methyltransferases - chemistry ; tRNA Methyltransferases - genetics ; tRNA Methyltransferases - metabolism
É parte de: Nucleic acids research, 2020-12, Vol.48 (21), p.12310-12325
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The authors wish it to be known that, in their opinion, the first two and last two authors should be regarded respectively as Joint First Authors and Joint Last Authors.
Descrição: The Mtq2-Trm112 methyltransferase modifies the eukaryotic translation termination factor eRF1 on the glutamine side chain of a universally conserved GGQ motif that is essential for release of newly synthesized peptides. Although this modification is found in the three domains of life, its exact role in eukaryotes remains unknown. As the deletion of MTQ2 leads to severe growth impairment in yeast, we have investigated its role further and tested its putative involvement in ribosome biogenesis. We found that Mtq2 is associated with nuclear 60S subunit precursors, and we demonstrate that its catalytic activity is required for nucleolar release of pre-60S and for efficient production of mature 5.8S and 25S rRNAs. Thus, we identify Mtq2 as a novel ribosome assembly factor important for large ribosomal subunit formation. We propose that Mtq2-Trm112 might modify eRF1 in the nucleus as part of a quality control mechanism aimed at proof-reading the peptidyl transferase center, where it will subsequently bind during translation termination.
Editor: England: Oxford University Press
Idioma: Inglês

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The catalytic activity of the translation termination factor methyltransferase Mtq2-Trm112 complex is required for large ribosomal subunit biogenesis

Lacoux, Caroline ; Wacheul, Ludivine ; Saraf, Kritika ; Pythoud, Nicolas ; Huvelle, Emmeline ; Figaro, Sabine ; Graille, Marc ; Carapito, Christine ; Lafontaine, Denis L J ; Heurgué-Hamard, Valérie

England: Oxford University Press

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