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Amino acid analysis methods and protocols

Michail A Alterman; Peter Hunziker

New York Humana Press c2012

Localização: CQ - Conjunto das Químicas    (574.19245 A517 e.2 )(Acessar)

  • Título:
    Amino acid analysis methods and protocols
  • Autor: Michail A Alterman; Peter Hunziker
  • Assuntos: Amino acids -- Analysis -- Laboratory manuals; AMINOÁCIDOS (ANÁLISE)
  • Notas: Includes bibliographical references and index.
  • Descrição: Rapid LC-MS/MS profiling of protein amino acids and metabolically related compounds for large-scale assessment of metabolic phenotypes -- Combination of an AccQ.Tag-ultra performance liquid chromatographic method with tandem mass spectrometry for the analysis of amino acids -- Isotope dilution liquid chromatography-tandem mass spectrometry for quantitative amino acid analysis -- Analysis of underivatized amino acids: Zwitterionic hydrophilic interaction chromatography combined with triple quadrupole tandem mass spectrometry -- Amino acid analysis via LC-MS method after derivatization with quaternary phosphonium -- Amino acid analysis by hydrophilic interaction chromatography coupled with isotope dilution mass spectrometry -- A universal HPLC-MS method to determine the stereochemistry of common and unusual amino acids -- Amino acid analysis by capillary electrophoresis-mass spectrometry -- New advances in amino acid profiling by capillary electrophoresis-electrospray ionization-mass spectrometry -- Optimal conditions for the direct RP-HPLC determination of underivatized amino acids with online multiple detection -- Absolute quantitation of proteins by acid hydrolysis combined with amino acid detection by mass spectrometry -- Amino acid analysis by means of MALDI TOF mass spectrometry or MALDI TOF/TOF tandem mass spectrometry -- Heptafluorobutyl chloroformate-based sample preparation protocol for chiral and nonchiral amino acid analysis by gas chromatography -- The EZ:Faast family of amino acid analysis kits: Application of the GC-FID kit for rapid determination of plasma tryptophan and other amino acids -- Amino acid analysis in physiological samples by GC-MS with propyl chloroformate derivatization and iTRAQ-LC-MS/MS -- Automated analysis of primary amino acids in plasma by high-performance liquid chromatography -- RP-LC of phenylthiocarbamyl amino acid adducts in plasma acetonitrile extracts: Use of multiple internal standards and variable wavelength UV
    detection -- -- Quantification of underivatised amino acids on dry blood spot, plasma, and urine by HPLC-ESI-MS/MS -- Capillary electrophoresis of free amino acids in physiological fluids without derivatization employing direct or indirect absorbance detection -- Measurement of 3-nitro-tyrosine in human plasma and urine by gas chromatography-tandem mass spectrometry -- Analysis of hydroxyproline in collagen hydrolysates -- Innovative and rapid procedure for 4-hydroxyproline determination in meat-based foods -- Multiple reaction monitoring for the accurate quantification of amino acids: Using hydroxyproline to estimate collagen content -- Sequential injection chromatography for fluorimetric determination of intracellular amino acids in marine microalgae -- Direct analysis of underivatized amino acids in plant extracts by LC-MS-MS -- Wheat gluten amino acid analysis by high-performance anion-exchange chromatography with integrated pulsed amperometric detection -- Preparative HPLC separation of underivatized amino acids for isotopic analysis -- Quantification of amino acids in a single cell by microchip electrophoresis with chemiluminescence detection
  • Títulos relacionados: Série:Methods in molecular biology (Clifton, N.J.) v. 828; Springer protocols
  • Editor: New York Humana Press
  • Data de criação/publicação: c2012
  • Formato: xiv, 363 p ill. (some col.) 26 cm.
  • Idioma: Inglês

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