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Using the mini-VIDAS® Easy Salmonella protocol to assess contamination in transitional and coastal waters

Rodrigues, M. J. ; Martins, K. ; Garcia, D. ; Ferreira, S. M. F. ; Gonçalves, S. C. ; Mendes, S. ; Lemos, M. F. L.

Archives of microbiology, 2016-07, Vol.198 (5), p.483-487 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

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Título:
Using the mini-VIDAS® Easy Salmonella protocol to assess contamination in transitional and coastal waters
Autor: Rodrigues, M. J. ; Martins, K. ; Garcia, D. ; Ferreira, S. M. F. ; Gonçalves, S. C. ; Mendes, S. ; Lemos, M. F. L.
Assuntos: Bacteriological Techniques - economics ; Bacteriological Techniques - methods ; Bacteriological Techniques - standards ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Ecology ; Life Sciences ; Microbial Ecology ; Microbiology ; Salmonella - isolation & purification ; Salmonella - physiology ; Seawater - microbiology ; Sensitivity and Specificity ; Short Communication ; Water Microbiology
É parte de: Archives of microbiology, 2016-07, Vol.198 (5), p.483-487
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Classical methodologies for Salmonella detection may be too long in time to assure public safety. Presently, one of the fastest assays for Salmonella detection using the mini-VIDAS ® system is the Easy Salmonella protocol. This assay, developed for food matrixes analysis, was here assessed for the applicability on the detection of these bacteria in transitional and saltwaters. The presence of Salmonella was detected in 4.2 % of the samples studied. In these transitional waters, the proposed protocol presented an efficiency of 79.1 %, due to a high false positive rate (20.8 %), and a false negative rate of 0 %—implying reducing analysis time, the use of enrichment broths, and making it more cost effective. Despite the multitude of samples nature, the method here described revealed to be an efficient and promising tool for transitional waters analysis.
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

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Using the mini-VIDAS® Easy Salmonella protocol to assess contamination in transitional and coastal waters

Rodrigues, M. J. ; Martins, K. ; Garcia, D. ; Ferreira, S. M. F. ; Gonçalves, S. C. ; Mendes, S. ; Lemos, M. F. L.

Berlin/Heidelberg: Springer Berlin Heidelberg

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