Language:

High-throughput sequencing of black pepper root transcriptome

Gordo, Sheila M C ; Pinheiro, Daniel G ; Moreira, Edith C O ; Rodrigues, Simone M ; Poltronieri, Marli C ; de Lemos, Oriel F ; da Silva, Israel Tojal ; Ramos, Rommel T J ; Silva, Artur ; Schneider, Horacio ; Silva, Jr, Wilson A ; Sampaio, Iracilda ; Darnet, Sylvain

BMC plant biology, 2012-09, Vol.12 (1), p.168-168, Article 168 [Peer Reviewed Journal]

England: BioMed Central Ltd

Full text available

Citations Cited by

Actions
1. Add to e-Shelf
2. Remove from e-Shelf
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
High-throughput sequencing of black pepper root transcriptome
Author: Gordo, Sheila M C ; Pinheiro, Daniel G ; Moreira, Edith C O ; Rodrigues, Simone M ; Poltronieri, Marli C ; de Lemos, Oriel F ; da Silva, Israel Tojal ; Ramos, Rommel T J ; Silva, Artur ; Schneider, Horacio ; Silva, Jr, Wilson A ; Sampaio, Iracilda ; Darnet, Sylvain
Subjects: Accuracy ; Angiosperms ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis thaliana ; Cooking ; Crops ; Data processing ; Databases, Genetic ; DNA sequencing ; Food ; Foot rot ; Fusarium solani ; Gene expression ; Gene Expression Profiling ; Gene Library ; Genetic aspects ; Genetic diversity ; Genetic transcription ; Genetics ; Genome, Plant ; Health aspects ; High-Throughput Nucleotide Sequencing ; Homology ; Infection ; Microsatellite Repeats ; Molecular Sequence Annotation ; Nucleotide sequencing ; Pathogens ; Peppers ; Phylogenetics ; Phylogeny ; Physiological aspects ; Piper nigrum ; Piper nigrum - genetics ; Piper nigrum - metabolism ; Plant breeding ; Plant genetics ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Roots - genetics ; Plant Roots - metabolism ; Preservation ; Proteins ; Proteome - genetics ; Proteome - metabolism ; RNA ; RNA sequencing ; RNA, Plant - genetics ; RNA, Plant - metabolism ; Root rot ; Roots (Botany) ; Sequence Analysis, RNA ; Sequence Homology, Amino Acid ; Spices ; Transcriptome
Is Part Of: BMC plant biology, 2012-09, Vol.12 (1), p.168-168, Article 168
Notes: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
Description: Black pepper (Piper nigrum L.) is one of the most popular spices in the world. It is used in cooking and the preservation of food and even has medicinal properties. Losses in production from disease are a major limitation in the culture of this crop. The major diseases are root rot and foot rot, which are results of root infection by Fusarium solani and Phytophtora capsici, respectively. Understanding the molecular interaction between the pathogens and the host's root region is important for obtaining resistant cultivars by biotechnological breeding. Genetic and molecular data for this species, though, are limited. In this paper, RNA-Seq technology has been employed, for the first time, to describe the root transcriptome of black pepper. The root transcriptome of black pepper was sequenced by the NGS SOLiD platform and assembled using the multiple-k method. Blast2Go and orthoMCL methods were used to annotate 10338 unigenes. The 4472 predicted proteins showed about 52% homology with the Arabidopsis proteome. Two root proteomes identified 615 proteins, which seem to define the plant's root pattern. Simple-sequence repeats were identified that may be useful in studies of genetic diversity and may have applications in biotechnology and ecology. This dataset of 10338 unigenes is crucially important for the biotechnological breeding of black pepper and the ecogenomics of the Magnoliids, a major group of basal angiosperms.
Publisher: England: BioMed Central Ltd
Language: English

Links

View this record in MEDLINE/PubMed

Back to results list

Result 1 Next Go to Next page

Implemented by: Social Network Logos:

High-throughput sequencing of black pepper root transcriptome

Gordo, Sheila M C ; Pinheiro, Daniel G ; Moreira, Edith C O ; Rodrigues, Simone M ; Poltronieri, Marli C ; de Lemos, Oriel F ; da Silva, Israel Tojal ; Ramos, Rommel T J ; Silva, Artur ; Schneider, Horacio ; Silva, Jr, Wilson A ; Sampaio, Iracilda ; Darnet, Sylvain

England: BioMed Central Ltd

Searching Remote Databases, Please Wait