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Biolumix Inc.; Patent Application Titled "Optical Method and Device for the Detection and Enumeration of Microorganisms" Published Online

Biotech Business Week, 2014, p.144

Atlanta: NewsRx

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  • Título:
    Biolumix Inc.; Patent Application Titled "Optical Method and Device for the Detection and Enumeration of Microorganisms" Published Online
  • É parte de: Biotech Business Week, 2014, p.144
  • Descrição: A novel and practical approach of culturing and monitoring microorganisms (bacteria, yeasts and molds) in test samples in the presence of interfering materials has been developed and successfully commercialized utilizing optical indicator substrates. One such product has been demonstrated by Turner, et al. (U.S. Pat. No. 4,945,060), Calandra, et al. (U.S. Pat. No. 5,094,955), Thorpe, et al. (U.S. Pat. No. 5,162,229), Di Guiseppi, et al. (U.S. Pat. No. 5,164,796) and Turner, et al. (U.S. Pat. No. 5,217,876). The basic principle of this device is to affix a disposable sensor to the interior surface of a transparent container that can monitor pH changes in the liquid media or the production of CO.sub.2 when the microorganisms grow and metabolize. The sensor comprises a solid composition or membrane with an indicator substrate immobilized on or within it. The sensor is placed flush against the inside surface of a container, such that the indicator substrate is visible from outside, and sealed to prevent the interfering compounds from getting between it and the container surface. In these embodiments the sensor is separated from the specimen and its growth media by a membrane or solid layer that permits the passage of gas molecules but prevents passage of ions. These devices are therefore characterized by two distinctive phases: (a) liquid phase that includes the growth media where the specimen is incubated and (b) solid phase in which the indicator substrate is embedded. In these devices, no growth media is present in the solid phase and no indicator substrate is present in the media. Practically, since sensors are based upon diffusion of CO.sub.2 gas (U.S. Pat. No. 5,217,876), they do require that the container is sealed during the incubation time so that the generated gas is pressurized through the sensor and cannot escape the container (U.S. Pat. No. 4,945,060). Consequently, these devices are limited to the determination of presence or absence of microorganisms in the tested samples which is adequate for clinical and sterility tests. Due to the solid nature of the sensor, the diffusion rate of the metabolites to the sensor is quite slow and may not be consistent for duplicate samples and, consequently, is inadequate for enumeration tests. The clear advantage of these devices is that they can be thermally sterilized (e.g., using autoclave at 121.degree.
  • Editor: Atlanta: NewsRx
  • Idioma: Inglês
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