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Detection of PCR inhibition in food and feed with a synthetic plasmid

Sovová, Tereza ; Křížová, Barbora ; Drábková, Lenka ; Ovesná, Jaroslava

Czech Journal of Food Sciences, 2017-01, Vol.35 (2), p.160-164 [Periódico revisado por pares]

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Título:
Detection of PCR inhibition in food and feed with a synthetic plasmid
Autor: Sovová, Tereza ; Křížová, Barbora ; Drábková, Lenka ; Ovesná, Jaroslava
Assuntos: Chloroplasts ; Cranberries ; Deoxyribonucleic acid ; DNA ; dna isolation ; Food ; food analysis ; food quality ; Homology ; Meat ; Nucleotide sequence ; Optimization ; pcr inhibitor ; Plasmids ; Polymerase chain reaction ; tRNA Leu
É parte de: Czech Journal of Food Sciences, 2017-01, Vol.35 (2), p.160-164
Descrição: We present a successful use of the plasmid inhibition detection and DNA isolation protocol optimisation for four food/feed samples in qPCR analysis of the sequence coding for chloroplast tRNA-Leu: two meat meal samples and two samples made of cranberries (jam and dried fruit). The quantitative real-time polymerase chain reaction (qPCR) can be inhibited by various substances and the DNA content in the sample can be underestimated. It is necessary to identify the PCR inhibition and choose an optimal DNA isolation protocol to correctly evaluate the sample. In a previous study, we have developed an assay using plasmid DNA carrying a non-homologous random sequence identifying possible inhibitors in qPCR in food/feed samples. The plasmid assay allowed to effectively reveal the PCR inhibition in all of the different sample matrices and to choose an optimal DNA isolation protocol.
Editor: Prague: Czech Academy of Agricultural Sciences (CAAS)
Idioma: Inglês;Tcheco;Eslovaco

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