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Dot Immunobinding Assay for the Rapid Serodetection of IScedosporium/I/ILomentospora/I in Cystic Fibrosis Patients

Ma ; Antoran, Aitziber ; Areitio, Maialen ; Aparicio-Fernandez, Leire ; Martín-Gómez, María Teresa ; Fernandez, Roberto ; Astigarraga, Egoitz ; Barreda-Gómez, Gabriel ; Schwarz, Carsten ; Rickerts, Volker ; Hernando, Fernando L ; Rementeria, Aitor ; Buldain, Idoia ; Ramirez-Garcia, Andoni

Journal of fungi (Basel), 2023-01, Vol.9 (2) [Periódico revisado por pares]

MDPI AG

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  • Título:
    Dot Immunobinding Assay for the Rapid Serodetection of IScedosporium/I/ILomentospora/I in Cystic Fibrosis Patients
  • Autor: Ma ; Antoran, Aitziber ; Areitio, Maialen ; Aparicio-Fernandez, Leire ; Martín-Gómez, María Teresa ; Fernandez, Roberto ; Astigarraga, Egoitz ; Barreda-Gómez, Gabriel ; Schwarz, Carsten ; Rickerts, Volker ; Hernando, Fernando L ; Rementeria, Aitor ; Buldain, Idoia ; Ramirez-Garcia, Andoni
  • Assuntos: Cystic fibrosis ; Fungal antigens ; Immunoglobulin G
  • É parte de: Journal of fungi (Basel), 2023-01, Vol.9 (2)
  • Descrição: The detection of Scedosporium/Lomentospora is still based on non-standardized low-sensitivity culture procedures. This fact is particularly worrying in patients with cystic fibrosis (CF), where these fungi are the second most common filamentous fungi isolated, because a poor and delayed diagnosis can worsen the prognosis of the disease. To contribute to the discovery of new diagnostic strategies, a rapid serological dot immunobinding assay (DIA) that allows the detection of serum IgG against Scedosporium/Lomentospora in less than 15 min was developed. A crude protein extract from the conidia and hyphae of Scedosporium boydii was employed as a fungal antigen. The DIA was evaluated using 303 CF serum samples (162 patients) grouped according to the detection of Scedosporium/Lomentospora in the respiratory sample by culture, obtaining a sensitivity and specificity of 90.48% and 79.30%, respectively; positive and negative predictive values of 54.81% and 96.77%, and an efficiency of 81.72%. The clinical factors associated with the results were also studied using a univariate and a multivariate analysis, which showed that Scedosporium/Lomentospora positive sputum, elevated anti-Aspergillus serum IgG and chronic Pseudomonas aeruginosa infection were significantly associated with a positive result in DIA, while Staphylococcus aureus positive sputum showed a negative association. In conclusion, the test developed can offer a complementary, rapid, simple and sensitive method to contribute to the diagnosis of Scedosporium/Lomentospora in patients with CF.
  • Editor: MDPI AG
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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