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SUPEROVULATION WITH PITUITARY GONADOTROPHINS METHOD FOR OBTAINING MEIOTIC METAPHASE FIGURES IN HUMAN OVA

Jagiello, Georgiana ; Karnicki, Jadwiga ; Ryan, RobertJ

The Lancet (British edition), 1968-01, Vol.291 (7535), p.178-180 [Periódico revisado por pares]

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Título:
SUPEROVULATION WITH PITUITARY GONADOTROPHINS METHOD FOR OBTAINING MEIOTIC METAPHASE FIGURES IN HUMAN OVA
Autor: Jagiello, Georgiana ; Karnicki, Jadwiga ; Ryan, RobertJ
Assuntos: Biopsy ; Children ; Chromosomes ; Cytomegalovirus ; Follicle-stimulating hormone ; Follicles ; Genetic counseling ; Genetic screening ; Gonadotropins ; Laparotomy ; Meiosis ; Menstrual cycle ; Menstruation ; Metaphase ; Mosaicism ; Ovaries ; Ovulation ; Patients ; Physiology ; Pituitary ; Pituitary (anterior)
É parte de: The Lancet (British edition), 1968-01, Vol.291 (7535), p.178-180
Descrição: Ova in the germinal-vesicle stage and first meiotic metaphase were obtained from the ovaries of women in whom superovulation had been induced. Patients were given a series of injections of pituitary follicle-stimulating hormone (F.S.H.) during the follicular phase of their menstrual cycle. On the 9th day of the cycle an ovulating stimulus, human chorionic gonadotrophin (H.C.G.), was given, and wedges from each ovary were removed next day for biopsy. Ova were harvested from the largest follicles. Some of these were processed for immediate cytogenetic examination, and the remainder were incubated for about 24 hours to obtain later meiotic stages. Information obtained with this method provides a valuable basis for genetic counselling of mothers with somatic chromosome mosaicism who have borne abnormal children.
Editor: London: Elsevier Ltd
Idioma: Inglês

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