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Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set

Kurome, Mayuko ; Geistlinger, Ludwig ; Kessler, Barbara ; Zakhartchenko, Valeri ; Klymiuk, Nikolai ; Wuensch, Annegret ; Richter, Anne ; Baehr, Andrea ; Kraehe, Katrin ; Burkhardt, Katinka ; Flisikowski, Krzysztof ; Flisikowska, Tatiana ; Merkl, Claudia ; Landmann, Martina ; Durkovic, Marina ; Tschukes, Alexander ; Kraner, Simone ; Schindelhauer, Dirk ; Petri, Tobias ; Kind, Alexander ; Nagashima, Hiroshi ; Schnieke, Angelika ; Zimmer, Ralf ; Wolf, Eckhard

BMC biotechnology, 2013-05, Vol.13 (1), p.43-43, Article 43 [Periódico revisado por pares]

England: BioMed Central Ltd

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Título:
Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set
Autor: Kurome, Mayuko ; Geistlinger, Ludwig ; Kessler, Barbara ; Zakhartchenko, Valeri ; Klymiuk, Nikolai ; Wuensch, Annegret ; Richter, Anne ; Baehr, Andrea ; Kraehe, Katrin ; Burkhardt, Katinka ; Flisikowski, Krzysztof ; Flisikowska, Tatiana ; Merkl, Claudia ; Landmann, Martina ; Durkovic, Marina ; Tschukes, Alexander ; Kraner, Simone ; Schindelhauer, Dirk ; Petri, Tobias ; Kind, Alexander ; Nagashima, Hiroshi ; Schnieke, Angelika ; Zimmer, Ralf ; Wolf, Eckhard
Assuntos: Analysis ; Animal experimentation ; Animal genetic engineering ; Animals ; Animals, Genetically Modified - genetics ; Animals, Genetically Modified - physiology ; Artificial chromosomes ; Biomedical research ; Birth ; Blastocyst - physiology ; Breeding of animals ; Bulk molding compounds ; Cloning ; Cloning, Molecular ; Comparative analysis ; Data Interpretation, Statistical ; Efficiency ; Embryonic development ; Embryos ; Experiments ; Female ; Gene Knockout Techniques ; Generalized linear models ; Genetic engineering ; Genetic modification ; Genetically modified animals ; Hogs ; Male ; Mathematical models ; Medical research ; Medicine, Experimental ; Nuclear transfer (Cloning) ; Nuclear Transfer Techniques - statistics & numerical data ; Pregnancy ; Seasons ; Stem cells ; Stillbirth ; Strategy ; Studies ; Swine ; Swine - genetics ; Swine - physiology
É parte de: BMC biotechnology, 2013-05, Vol.13 (1), p.43-43, Article 43
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Somatic cell nuclear transfer (SCNT) using genetically engineered donor cells is currently the most widely used strategy to generate tailored pig models for biomedical research. Although this approach facilitates a similar spectrum of genetic modifications as in rodent models, the outcome in terms of live cloned piglets is quite variable. In this study, we aimed at a comprehensive analysis of environmental and experimental factors that are substantially influencing the efficiency of generating genetically engineered pigs. Based on a considerably large data set from 274 SCNT experiments (in total 18,649 reconstructed embryos transferred into 193 recipients), performed over a period of three years, we assessed the relative contribution of season, type of genetic modification, donor cell source, number of cloning rounds, and pre-selection of cloned embryos for early development to the cloning efficiency. 109 (56%) recipients became pregnant and 85 (78%) of them gave birth to offspring. Out of 318 cloned piglets, 243 (76%) were alive, but only 97 (40%) were clinically healthy and showed normal development. The proportion of stillborn piglets was 24% (75/318), and another 31% (100/318) of the cloned piglets died soon after birth. The overall cloning efficiency, defined as the number of offspring born per SCNT embryos transferred, including only recipients that delivered, was 3.95%. SCNT experiments performed during winter using fetal fibroblasts or kidney cells after additive gene transfer resulted in the highest number of live and healthy offspring, while two or more rounds of cloning and nuclear transfer experiments performed during summer decreased the number of healthy offspring. Although the effects of individual factors may be different between various laboratories, our results and analysis strategy will help to identify and optimize the factors, which are most critical to cloning success in programs aiming at the generation of genetically engineered pig models.
Editor: England: BioMed Central Ltd
Idioma: Inglês

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Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set

England: BioMed Central Ltd

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