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Mice lacking CCAAt/enhancer-binding protein-alpha show hyperproliferation of alveolar type II cells and increased surfactant protein mRNAs

Sugahara, K ; Iyama, K I ; Kimura, T ; Sano, K ; Darlington, G J ; Akiba, T ; Takiguchi, M

Cell and tissue research, 2001-10, Vol.306 (1), p.57-63 [Periódico revisado por pares]

Germany

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Título:
Mice lacking CCAAt/enhancer-binding protein-alpha show hyperproliferation of alveolar type II cells and increased surfactant protein mRNAs
Autor: Sugahara, K ; Iyama, K I ; Kimura, T ; Sano, K ; Darlington, G J ; Akiba, T ; Takiguchi, M
Assuntos: Animals ; CCAAT-Enhancer-Binding Protein-alpha - deficiency ; CCAAT-Enhancer-Binding Protein-alpha - genetics ; Cell Division ; Immunohistochemistry ; In Situ Hybridization ; Mice ; Mice, Knockout ; Proteolipids - genetics ; Pulmonary Alveoli - metabolism ; Pulmonary Alveoli - pathology ; Pulmonary Surfactant-Associated Protein A ; Pulmonary Surfactant-Associated Proteins ; Pulmonary Surfactants - genetics ; RNA, Messenger - genetics ; RNA, Messenger - metabolism
É parte de: Cell and tissue research, 2001-10, Vol.306 (1), p.57-63
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: The lung-specific surfactant proteins (SP) are essential for normal respiratory function. Transcription factors may play an important role in the regulation of surfactant proteins. The CCAAT/enhancer-binding protein (C/EBP) family consists of transcription factors that can stimulate expression of genes in lipid-metabolizing epithelial cells. C/EBPalpha-deficient mice have been shown to exhibit abnormal pulmonary histopathology. Recently, we demonstrated that C/EBP family members are differentially expressed in alveolar type II cell proliferation and in pulmonary fibrosis. In the present study, to investigate whether the C/EBP family would be involved in the regulation of surfactant proteins, we examined the protein expression of SP-A, and SP-C, and mRNA expression of SP-A, SP-B, and SP-C in the lungs from newborn C/EBPalpha-deficient mice. Using immunohistochemistry, we demonstrated that positive cells for SP-C, specific to alveolar type II cells, in the lungs were more abundant in the newborn C/EBPalpha-deficient mice than in control mice, which suggests the hyperproliferation of alveolar type II cells in the lungs of the C/EBPalpha-deficient mice. In situ hybridization analysis revealed that expression of SP-A, SP-B, and SP-C mRNAs were increased in the lungs of newborn C/EBPalpha-deficient mice. Northern blot analysis revealed that surfactant protein mRNAs were also increased. Thus, these results suggest that C/EBPalpha may play a key role in the proliferation of alveolar type II cells and the regulation of genes of surfactant protein.
Editor: Germany
Idioma: Inglês

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Mice lacking CCAAt/enhancer-binding protein-alpha show hyperproliferation of alveolar type II cells and increased surfactant protein mRNAs

Sugahara, K ; Iyama, K I ; Kimura, T ; Sano, K ; Darlington, G J ; Akiba, T ; Takiguchi, M

Germany

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