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1,25-Dihydroxyvitamin D3 inhibits antigen-induced T cell activation

Bhalla, AK ; Amento, EP ; Serog, B ; Glimcher, LH

The Journal of immunology (1950), 1984-10, Vol.133 (4), p.1748-1754 [Peer Reviewed Journal]

Bethesda, MD: Am Assoc Immnol

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Title:
1,25-Dihydroxyvitamin D3 inhibits antigen-induced T cell activation
Author: Bhalla, AK ; Amento, EP ; Serog, B ; Glimcher, LH
Subjects: Analysis of the immune response. Humoral and cellular immunity ; Animals ; Antigens - immunology ; Biological and medical sciences ; Calcitriol - pharmacology ; Cell interactions ; Epitopes ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Hybridomas - immunology ; Immunobiology ; Immunosuppressive Agents - pharmacology ; Interleukin-2 - biosynthesis ; Kinetics ; Lymph Nodes - cytology ; Lymphocyte Activation - drug effects ; Lymphocyte Culture Test, Mixed ; Mice ; Mice, Inbred BALB C ; Receptors, Calcitriol ; Receptors, Steroid - analysis ; T-Lymphocytes - immunology ; T-Lymphocytes - metabolism
Is Part Of: The Journal of immunology (1950), 1984-10, Vol.133 (4), p.1748-1754
Notes: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Description: The proliferative response of murine spleen and thymus cells to antigen but not to lectin was inhibited by the active metabolite of vitamin D3, 1,25-(OH)2D3. To directly examine the effect of 1,25-(OH)2D3 on T cell activation in the absence of other complicating interactions, we utilized a panel of cloned Ia-restricted T cell hybridomas that secrete IL 2 on activation by cloned Ia-bearing stimulator cells (TA3) or when stimulated by mitogen. Physiologic concentrations of 1,25-(OH)2D3 (0.01 to 0.1 nm) inhibited the antigen-induced secretion of IL 2 by several of these T cell hybridomas. This inhibition was dependent on the concentration of the free hormone and could be overcome by increasing the number of Ia-bearing stimulator cells used. Pretreatment of the T hybridoma but not the TA3 stimulator cell with 1,25-(OH)2D3 resulted in inhibition of activation. These results are consistent with the finding that specific 1,25-(OH)2D3 receptors are present on the T cell hybridomas but are lacking in TA3 cells. 1,25-(OH)2D3 failed, however, to inhibit the activation of the T cell hybridomas by lectin or by an anti-Thy-1 antibody. These findings suggest that 1,25-(OH)2D3 may be interfering with early events of antigen-induced T cell activation, perhaps by hindering T cell recognition of the relevant antigen on stimulator cell surfaces. This system should prove useful in studying the molecular mechanisms by which 1,25-(OH)2D3 acts to inhibit T cell activation and subsequent IL 2 production.
Publisher: Bethesda, MD: Am Assoc Immnol
Language: English

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1,25-Dihydroxyvitamin D3 inhibits antigen-induced T cell activation

Bhalla, AK ; Amento, EP ; Serog, B ; Glimcher, LH

Bethesda, MD: Am Assoc Immnol

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