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Evaluation of the Cytotoxic Effect of Pd2Spm against Prostate Cancer through Vibrational Microspectroscopies

Laginha, Raquel C. ; Martins, Clara B. ; Brandão, Ana L. C. ; Marques, Joana ; Marques, M. Paula M. ; Batista de Carvalho, Luís A. E. ; Santos, Inês P. ; Batista de Carvalho, Ana L. M.

International journal of molecular sciences, 2023-02, Vol.24 (3), p.1888 [Revista revisada por pares]

Basel: MDPI AG

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Título:
Evaluation of the Cytotoxic Effect of Pd2Spm against Prostate Cancer through Vibrational Microspectroscopies
Autor: Laginha, Raquel C. ; Martins, Clara B. ; Brandão, Ana L. C. ; Marques, Joana ; Marques, M. Paula M. ; Batista de Carvalho, Luís A. E. ; Santos, Inês P. ; Batista de Carvalho, Ana L. M.
Materias: Androgens ; Biopsy ; Cancer therapies ; Cell cycle ; Cell growth ; Chemotherapy ; Cisplatin ; Conformation ; Cytotoxicity ; Deoxyribonucleic acid ; DNA ; DNA structure ; Drugs ; Evaluation ; Fourier transforms ; FTIR microspectroscopy ; Infrared analysis ; Lipids ; Medical screening ; Metabolism ; Metastasis ; palladium(II) ; Polyamines ; Principal components analysis ; Prostate cancer ; Proteins ; Raman microspectroscopy ; Spectrum analysis ; Spermine ; Testicular cancer
Es parte de: International journal of molecular sciences, 2023-02, Vol.24 (3), p.1888
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
These authors contributed equally to this work.
Descripción: Regarding the development of new antineoplastic agents, with a view to assess the selective antitumoral potential which aims at causing irreversible damage to cancer cells while preserving the integrity of their healthy counterparts, it is essential to evaluate the cytotoxic effects in both healthy and malignant human cell lines. In this study, a complex with two Pd(II) centers linked by the biogenic polyamine spermine (Pd2Spm) was tested on healthy (PNT-2) and cancer (LNCaP and PC-3) prostate human cell lines, using cisplatin as a reference. To understand the mechanisms of action of both cisplatin and Pd2Spm at a molecular level, Fourier Transform Infrared (FTIR) and Raman microspectroscopies were used. Principal component analysis was applied to the vibrational data, revealing the major metabolic changes caused by each drug, which were found to rely on DNA, lipids, and proteins, acting as biomarkers of drug impact. The main changes were observed between the B-DNA native conformation and either Z-DNA or A-DNA, with a higher effect on lipids having been detected in the presence of cisplatin as compared to Pd2Spm. In turn, the Pd-agent showed a more significant impact on proteins.
Editor: Basel: MDPI AG
Idioma: Inglés

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Evaluation of the Cytotoxic Effect of Pd2Spm against Prostate Cancer through Vibrational Microspectroscopies

Laginha, Raquel C. ; Martins, Clara B. ; Brandão, Ana L. C. ; Marques, Joana ; Marques, M. Paula M. ; Batista de Carvalho, Luís A. E. ; Santos, Inês P. ; Batista de Carvalho, Ana L. M.

Basel: MDPI AG

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