Idioma:

Biochemical characterization of SARS-CoV-2 nucleocapsid protein

Zeng, Weihong ; Liu, Guangfeng ; Ma, Huan ; Zhao, Dan ; Yang, Yunru ; Liu, Muziying ; Mohammed, Ahmed ; Zhao, Changcheng ; Yang, Yun ; Xie, Jiajia ; Ding, Chengchao ; Ma, Xiaoling ; Weng, Jianping ; Gao, Yong ; He, Hongliang ; Jin, Tengchuan

Biochemical and biophysical research communications, 2020-06, Vol.527 (3), p.618-623 [Periódico revisado por pares]

United States: Elsevier Inc

Texto completo disponível

Citações Citado por

Enviar para

Título:
Biochemical characterization of SARS-CoV-2 nucleocapsid protein
Autor: Zeng, Weihong ; Liu, Guangfeng ; Ma, Huan ; Zhao, Dan ; Yang, Yunru ; Liu, Muziying ; Mohammed, Ahmed ; Zhao, Changcheng ; Yang, Yun ; Xie, Jiajia ; Ding, Chengchao ; Ma, Xiaoling ; Weng, Jianping ; Gao, Yong ; He, Hongliang ; Jin, Tengchuan
Assuntos: Antibodies, Viral - blood ; Antigenicity ; Betacoronavirus - chemistry ; Coronavirus Infections ; COVID-19 ; Humans ; Nucleic Acids ; Nucleocapsid protein ; Nucleocapsid Proteins - chemistry ; Pandemics ; Pneumonia, Viral ; Protein Binding ; Protein Multimerization ; Protein Structure, Tertiary ; SARS-CoV-2 ; SAXS ; Scattering, Small Angle ; Structure and function ; X-Ray Diffraction
É parte de: Biochemical and biophysical research communications, 2020-06, Vol.527 (3), p.618-623
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: The nucleocapsid (N) protein is an important antigen for coronavirus, which participate in RNA package and virus particle release. In this study, we expressed the N protein of SARS-CoV-2 and characterized its biochemical properties. Static light scattering, size exclusive chromatography, and small-angle X-ray scattering (SAXS) showed that the purified N protein is largely a dimer in solution. CD spectra showed that it has a high percentage of disordered region at room temperature while it was best structured at 55 °C, suggesting its structural dynamics. Fluorescence polarization assay showed it has non-specific nucleic acid binding capability, which raised a concern in using it as a diagnostic marker. Immunoblot assays confirmed the presence of IgA, IgM and IgG antibodies against N antigen in COVID-19 infection patients’ sera, proving the importance of this antigen in host immunity and diagnostics. •SARS-CoV-2 nucleocapsid protein is full of coils and highly disordered.•SARS-CoV-2 N protein forms a dimer by CTD-CTD interaction.•SARS-CoV-2 N protein can bind with non-specific nucleic acid with high affinity.•SARS-CoV-2 N protein can be a good antigen for serological test of COVID-19.
Editor: United States: Elsevier Inc
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Biochemical characterization of SARS-CoV-2 nucleocapsid protein

Zeng, Weihong ; Liu, Guangfeng ; Ma, Huan ; Zhao, Dan ; Yang, Yunru ; Liu, Muziying ; Mohammed, Ahmed ; Zhao, Changcheng ; Yang, Yun ; Xie, Jiajia ; Ding, Chengchao ; Ma, Xiaoling ; Weng, Jianping ; Gao, Yong ; He, Hongliang ; Jin, Tengchuan

United States: Elsevier Inc

Buscando em bases de dados remotas. Favor aguardar.