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Mdivi-1, mitochondrial fission inhibitor, impairs developmental competence and mitochondrial function of embryos and cells in pigs

YEON, Ji-Yeong ; MIN, Sung-Hun ; PARK, Hyo-Jin ; KIM, Jin-Woo ; LEE, Yong-Hee ; PARK, Soo-Yong ; JEONG, Pil-Soo ; PARK, Humdai ; LEE, Dong-Seok ; KIM, Sun-Uk ; CHANG, Kyu-Tae ; KOO, Deog-Bon

Journal of Reproduction and Development, 2015, Vol.61(2), pp.81-89 [Periódico revisado por pares]

Japan: THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

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Título:
Mdivi-1, mitochondrial fission inhibitor, impairs developmental competence and mitochondrial function of embryos and cells in pigs
Autor: YEON, Ji-Yeong ; MIN, Sung-Hun ; PARK, Hyo-Jin ; KIM, Jin-Woo ; LEE, Yong-Hee ; PARK, Soo-Yong ; JEONG, Pil-Soo ; PARK, Humdai ; LEE, Dong-Seok ; KIM, Sun-Uk ; CHANG, Kyu-Tae ; KOO, Deog-Bon
Assuntos: Animals ; Apoptosis - drug effects ; Apoptosis - physiology ; Death-Associated Protein Kinases - metabolism ; Embryo Culture Techniques ; Embryonic Development - drug effects ; Embryonic Development - physiology ; Embryos ; Fibroblast cells ; Mdivi-1 ; Membrane Potential, Mitochondrial - drug effects ; Membrane Potential, Mitochondrial - physiology ; Mitochondria ; Mitochondria - drug effects ; Mitochondria - metabolism ; Mitochondrial Dynamics - drug effects ; Mitochondrial Dynamics - physiology ; Original ; Pig ; Quinazolinones - pharmacology ; Reactive Oxygen Species - metabolism ; Swine
É parte de: Journal of Reproduction and Development, 2015, Vol.61(2), pp.81-89
Notas: J-Y Yeon and S-H Min contributed equally to this work.
Descrição: Mitochondria are highly dynamic organelles that undergo constant fusion/fission as well as activities orchestrated by large dynamin-related GTPases. These dynamic mitochondrial processes influence mitochondrial morphology, size and function. Therefore, this study was conducted to evaluate the effects of mitochondrial fission inhibitor, mdivi-1, on developmental competence and mitochondrial function of porcine embryos and primary cells. Presumptive porcine embryos were cultured in PZM-3 medium supplemented with mdivi-1 (0, 10 and 50 μM) for 6 days. Porcine fibroblast cells were cultured in growth medium with mdivi-1 (0 and 50 μM) for 2 days. Our results showed that the rate of blastocyst production and cell growth in the mdivi-1 (50 μM) treated group was lower than that of the control group (P < 0.05). Moreover, loss of mitochondrial membrane potential in the mdivi-1 (50 μM) treated group was increased relative to the control group (P < 0.05). Subsequent evaluation revealed that the intracellular levels of reactive oxygen species (ROS) and the apoptotic index were increased by mdivi-1 (50 μM) treatment (P < 0.05). Finally, the expression of mitochondrial fission-related protein (Drp 1) was lower in the embryos and cells in the mdivi-1-treated group than the control group. Taken together, these results indicate that mdivi-1 treatment may inhibit developmental competence and mitochondrial function in porcine embryos and primary cells.
Editor: Japan: THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT
Idioma: Inglês

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Mdivi-1, mitochondrial fission inhibitor, impairs developmental competence and mitochondrial function of embryos and cells in pigs

YEON, Ji-Yeong ; MIN, Sung-Hun ; PARK, Hyo-Jin ; KIM, Jin-Woo ; LEE, Yong-Hee ; PARK, Soo-Yong ; JEONG, Pil-Soo ; PARK, Humdai ; LEE, Dong-Seok ; KIM, Sun-Uk ; CHANG, Kyu-Tae ; KOO, Deog-Bon

Japan: THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

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