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Modification of mitochondrial function, cytoplasmic lipid content and cryosensitivity of bovine embryos by resveratrol

ABE, Takahito ; KAWAHARA-MIKI, Ryouka ; HARA, Tomotaka ; NOGUCHI, Tatsuo ; HAYASHI, Takeshi ; SHIRASUNA, Koumei ; KUWAYAMA, Takehito ; IWATA, Hisataka

Journal of Reproduction and Development, 2017, Vol.63(5), pp.455-461 [Periódico revisado por pares]

Japan: THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

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Título:
Modification of mitochondrial function, cytoplasmic lipid content and cryosensitivity of bovine embryos by resveratrol
Autor: ABE, Takahito ; KAWAHARA-MIKI, Ryouka ; HARA, Tomotaka ; NOGUCHI, Tatsuo ; HAYASHI, Takeshi ; SHIRASUNA, Koumei ; KUWAYAMA, Takehito ; IWATA, Hisataka
Assuntos: AMP ; AMP-activated protein kinase ; Animals ; Blastocyst - cytology ; Blastocyst - drug effects ; Blastocyst - metabolism ; Blastocysts ; Cattle ; Cell culture ; Cell Survival - drug effects ; Cells, Cultured ; Cold tolerance ; Cryopreservation ; Culture Media ; Cytoplasm - chemistry ; Cytoplasm - drug effects ; Cytoplasm - metabolism ; Embryo Culture Techniques - veterinary ; Embryo, Mammalian - drug effects ; Embryo, Mammalian - physiology ; Embryonic Development - drug effects ; Embryos ; Female ; Fertilization in Vitro - veterinary ; Kinases ; Lipid ; Lipid metabolism ; Lipid Metabolism - drug effects ; Lipids ; Lipids - analysis ; Male ; Metabolism ; Mitochondria ; Mitochondria - drug effects ; Mitochondria - physiology ; Mitochondrial DNA ; NAD ; Original ; Oxidation ; Reactive oxygen species ; Resveratrol ; SIRT1 protein ; Somatic cells ; Stilbenes - pharmacology
É parte de: Journal of Reproduction and Development, 2017, Vol.63(5), pp.455-461
Notas: T Abe and R Kawahara-Miki contributed equally to this work.
Descrição: Resveratrol is a potent activator of NAD-dependent deacetyltransferase sirtuin-1 (SIRT1) and affects lipid metabolism and ATP generation in somatic cells. In the present study, the effects of supplementing culture medium with resveratrol on lipid metabolism, ATP generation, and cryosensitivity of bovine in vitro produced embryos were investigated. Bovine early cleaved-stage embryos were cultured in medium containing 0 or 0.5 µM resveratrol for 1 or 5 days. Resveratrol treatment for both 1 day and 5 days increased the expression levels of SIRT1 and phosphorylated AMP-activated protein kinase (pAMPK) in the embryos. Furthermore, resveratrol treatment was effective to increase ATP generation and reduce lipid content of the embryos. The effects of resveratrol treatment were diminished by the SIRT1 inhibitor “EX527”, and the reduced lipid content was reversed by treatment with etomoxir (a potent inhibitor of beta-oxidation). Blastocysts developed after resveratrol treatment showed low levels reactive oxygen species and increased cryotolerance. These results demonstrate that resveratrol improves in vitro development of bovine embryos, while reducing cytoplasmic lipid content through activation of beta-oxidation, thereby effective for production of bovine blastocysts with enhanced cryotolerance.
Editor: Japan: THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT
Idioma: Inglês

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Modification of mitochondrial function, cytoplasmic lipid content and cryosensitivity of bovine embryos by resveratrol

ABE, Takahito ; KAWAHARA-MIKI, Ryouka ; HARA, Tomotaka ; NOGUCHI, Tatsuo ; HAYASHI, Takeshi ; SHIRASUNA, Koumei ; KUWAYAMA, Takehito ; IWATA, Hisataka

Japan: THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

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