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Bean polygalacturonase-inhibiting protein expressed in transgenic Brassica napus inhibits polygalacturonase from its fungal pathogen Rhizoctonia solani

Akhgari, A.B., National Inst. of Genetic Engineering and Biotechnology,Tehran (Iran Islamic Republic) ; Motallebi, M., National Inst. of Genetic Engineering and Biotechnology,Tehran (Iran Islamic Republic) ; Zamani, M.R., National Inst. of Genetic Engineering and Biotechnology,Tehran (Iran Islamic Republic)

Plant protection science, 2012-01, Vol.48 (1), p.1-9 [Periódico revisado por pares]

Prague: Czech Academy of Agricultural Sciences (CAAS)

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Título:
Bean polygalacturonase-inhibiting protein expressed in transgenic Brassica napus inhibits polygalacturonase from its fungal pathogen Rhizoctonia solani
Autor: Akhgari, A.B., National Inst. of Genetic Engineering and Biotechnology,Tehran (Iran Islamic Republic) ; Motallebi, M., National Inst. of Genetic Engineering and Biotechnology,Tehran (Iran Islamic Republic) ; Zamani, M.R., National Inst. of Genetic Engineering and Biotechnology,Tehran (Iran Islamic Republic)
Assuntos: ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; AGENT PATHOGENE ; AGROBACTERIUM TUMEFACIENS ; bean ; Beans ; Brassica ; BRASSICA NAPUS ; Canola ; CHAMPIGNON ; CLONACION MOLECULAR ; CLONAGE MOLECULAIRE ; Damping ; ENZYME ACTIVITY ; ENZYME INHIBITORS ; EXPLANT ; EXPLANTES ; EXPLANTS ; EXPRESION GENICA ; EXPRESSION DES GENES ; FRIJOL (PHASEOLUS) ; FUNGI ; Fungicides ; GENE ; GENE EXPRESSION ; GENE TRANSFER ; GENES ; GENETIC ENGINEERING ; GENETIC TRANSFORMATION ; GENETIC VECTORS ; GENIE GENETIQUE ; GRAINE DE COLZA ; HARICOT COMMUN ; HONGOS ; http://www.fao.org/aos/agrovoc#c_1066 ; http://www.fao.org/aos/agrovoc#c_15974 ; http://www.fao.org/aos/agrovoc#c_24846 ; http://www.fao.org/aos/agrovoc#c_25499 ; http://www.fao.org/aos/agrovoc#c_2599 ; http://www.fao.org/aos/agrovoc#c_2604 ; http://www.fao.org/aos/agrovoc#c_27444 ; http://www.fao.org/aos/agrovoc#c_27503 ; http://www.fao.org/aos/agrovoc#c_27520 ; http://www.fao.org/aos/agrovoc#c_27527 ; http://www.fao.org/aos/agrovoc#c_27532 ; http://www.fao.org/aos/agrovoc#c_27619 ; http://www.fao.org/aos/agrovoc#c_28427 ; http://www.fao.org/aos/agrovoc#c_29529 ; http://www.fao.org/aos/agrovoc#c_3145 ; http://www.fao.org/aos/agrovoc#c_3214 ; http://www.fao.org/aos/agrovoc#c_3220 ; http://www.fao.org/aos/agrovoc#c_32817 ; http://www.fao.org/aos/agrovoc#c_33858 ; http://www.fao.org/aos/agrovoc#c_34079 ; http://www.fao.org/aos/agrovoc#c_4098 ; http://www.fao.org/aos/agrovoc#c_5630 ; http://www.fao.org/aos/agrovoc#c_5767 ; http://www.fao.org/aos/agrovoc#c_6259 ; IMMUNOBLOTTING ; INGENIERIA GENETICA ; INHIBICION ; INHIBIDORES DE ENZIMAS ; INHIBITEUR D'ENZYME ; INHIBITION ; KIDNEY BEANS ; Leaves ; MOLECULAR CLONING ; ORGANISMOS PATOGENOS ; PATHOGENS ; PCR ; pgip ; PHASEOLUS VULGARIS ; Plant extracts ; Plant protection ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; POLIGALACTURONASA ; POLYGALACTURONASE ; Polymerase chain reaction ; PROTEINAS ; PROTEINE ; PROTEINS ; Rape plants ; RAPESEED ; RHIZOCTONIA SOLANI ; Root rot ; SEMILLA DE COLZA ; TRANSFERENCIA DE GENES ; TRANSFERT DE GENE ; TRANSFORMACION GENETICA ; Transformation ; TRANSFORMATION GENETIQUE ; TRANSGENIC PLANTS ; VECTEUR GENETIQUE ; VECTORES GENETICOS
É parte de: Plant protection science, 2012-01, Vol.48 (1), p.1-9
Notas: F30
2012000275
H20
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
Descrição: Polygalacturonase-inhibiting proteins (PGIPs) selectively inhibit polygalacturonases (PGs) secreted by invading plant pathogenic fungi. The objective of the present research was to clone and introduce the pgip2 gene from Phaseolus vulgaris cv. Goli, with antifungal potential, into Brassica napus (R line Hyola 308) via Agrobacterium tumefaciens mediated transformation. Here we used a transgenic overexpression approach in order to investigate the inhibitory activity of the PGIP on the PG from Rhizoctonia solani. PGIP expression was determined in the functional inhibition assays against fungal PGs. Crude protein extracts prepared from transgenic canola leaves were found to inhibit the R. solani PG from 29% to 37% as compared to untransformed plants. The putative transgenic canola lines harbouring the pgip2 gene encoding polygalacturonase-inhibiting proteins were identified by polymerase chain reaction and Southern blot analysis.
Editor: Prague: Czech Academy of Agricultural Sciences (CAAS)
Idioma: Inglês

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Bean polygalacturonase-inhibiting protein expressed in transgenic Brassica napus inhibits polygalacturonase from its fungal pathogen Rhizoctonia solani

Prague: Czech Academy of Agricultural Sciences (CAAS)

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