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3D nuclear architecture distinguishes thyroid neoplastic histotypes

Pozzo, Aline Rangel

Biblioteca Digital de Teses e Dissertações da USP; Universidade de São Paulo; Bioinformática 2024-04-23

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  • Título:
    3D nuclear architecture distinguishes thyroid neoplastic histotypes
  • Autor: Pozzo, Aline Rangel
  • Orientador: Galante, Pedro Alexandre Favoretto
  • Assuntos: 3d-Sim; Diferenciação De Tipos Histológicos; Sequenciamento De Nova Geração; Neoplasia Folicular Não-Invasiva Da Tireoide Com Características Nucleares Papilífero-Similares; Perfil 3d De Telômeros; 3d-Sim; Papillary Thyroid Carcinoma; Next Generation Sequencing; Histotypes Differentiation; Telomeres Molecular Profile
  • Notas: Dissertação (Mestrado)
  • Descrição: Molecular markers can be diagnostic tools to support pathological analysis in thyroid neoplasms. However, because the same markers can be observed in some benign thyroid lesions, additional approaches are necessary to differentiate thyroid tumour subtypes, prevent overtreatment and tailor specific clinical management. This applies particularly to the recently described variant of thyroid cancer referred to as non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP). This variant has an estimated prevalence of 4.4% to 9.1% of all papillary thyroid carcinomas worldwide. We studied 60 thyroid lesions: 20 classical papillary thyroid carcinoma (CPTC), 20 follicular variants of PTC (FVPTC) and 20 NIFTP. We examined morphological and molecular features to identify parameters that can differentiate NIFTP from the other PTC subtypes. When blindly investigating the nuclear architecture of thyroid neoplasms, we observed that NIFTP has significantly longer telomeres than CPTC and FVPTC. Super-resolved 3D-structured illumination microscopy demonstrated that NIFTP is heterogeneous and that its nuclei contain more densely packed DNA and smaller interchromatin spaces than CPTC and FVPTC, a pattern that resembles normal thyroid tissue. These data are consistent with the observed indolent biological behaviour and favourable prognosis associated with NIFTP, which lacks BRAFV600E mutations. Of note, next-generation thyroid oncological panel sequencing could not distinguish the thyroid cancer histotypes in our study cohort. In summary, our data suggest that 3D nuclear architecture can be a powerful analytical tool to diagnose and guide the clinical management of NIFTP.
  • DOI: 10.11606/D.95.2024.tde-28052024-203350
  • Editor: Biblioteca Digital de Teses e Dissertações da USP; Universidade de São Paulo; Bioinformática
  • Data de criação/publicação: 2024-04-23
  • Formato: Adobe PDF
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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