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Erythropoietin and Erythropoietin Receptors in Human CNS Neurons, Astrocytes, Microglia, and Oligodendrocytes Grown in Culture

NAGAI, ATSUSHI ; NAKAGAWA, EIJI ; CHOI, HYUN B ; HATORI, KOZO ; KOBAYASHI, SHOTAI ; KIM, SEUNG U

Journal of neuropathology and experimental neurology, 2001-04, Vol.60 (4), p.386-392 [Periódico revisado por pares]

Hagerstown, MD: American Association of Neuropathologists, Inc

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Título:
Erythropoietin and Erythropoietin Receptors in Human CNS Neurons, Astrocytes, Microglia, and Oligodendrocytes Grown in Culture
Autor: NAGAI, ATSUSHI ; NAKAGAWA, EIJI ; CHOI, HYUN B ; HATORI, KOZO ; KOBAYASHI, SHOTAI ; KIM, SEUNG U
Assuntos: Astrocytes - cytology ; Astrocytes - drug effects ; Astrocytes - metabolism ; Biological and medical sciences ; Cells, Cultured ; Central Nervous System - cytology ; Central Nervous System - embryology ; Central Nervous System - metabolism ; Cytokines - pharmacology ; Enzyme-Linked Immunosorbent Assay ; Erythropoietin - biosynthesis ; Erythropoietin - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression - drug effects ; Humans ; Isolated neuron and nerve. Neuroglia ; Microglia - cytology ; Microglia - metabolism ; Neurons - cytology ; Neurons - drug effects ; Neurons - metabolism ; Oligodendroglia - cytology ; Oligodendroglia - metabolism ; Receptors, Erythropoietin - biosynthesis ; Receptors, Erythropoietin - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Tumor Necrosis Factor-alpha - pharmacology ; Vertebrates: nervous system and sense organs
É parte de: Journal of neuropathology and experimental neurology, 2001-04, Vol.60 (4), p.386-392
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Descrição: Erythropoietin (EPO) is a hematopoietic growth factor that stimulates proliferation and differentiation of erythroid precursor cells and is also known to exert neurotrophic activity in the central nervous system (CNS). However, little is known about expression of EPO and EPO receptor (EPOR) in human CNS tissues. In the present study, we investigated the effects of proinflammatory cytokines on EPO and EPOR expression in highly purified cultures of human neurons, astrocytes, microglia, and oligodendrocytes using reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). EPO mRNA was demonstrated only in human astrocytes, while EPOR expression was found in human neurons, astrocytes, and microglia. Neither EPO nor EPOR expression was found in oligodendrocytes. In human astrocytes, EPO mRNA and secreted EPO protein levels were downregulated after exposure to proinflammatory cytokines (IL-1β, IL-6, or TNF-α). In human neurons, TNF-α treatment markedly increased EPOR expression. These results suggest that proinflammatory cytokines regulate expression of EPO and EPOR in human neurons, astrocytes, and microglia and further facilitate interactions among different cell types in the human CNS.
Editor: Hagerstown, MD: American Association of Neuropathologists, Inc
Idioma: Inglês

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Erythropoietin and Erythropoietin Receptors in Human CNS Neurons, Astrocytes, Microglia, and Oligodendrocytes Grown in Culture

NAGAI, ATSUSHI ; NAKAGAWA, EIJI ; CHOI, HYUN B ; HATORI, KOZO ; KOBAYASHI, SHOTAI ; KIM, SEUNG U

Hagerstown, MD: American Association of Neuropathologists, Inc

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