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Micropatterned Silica Films with Nanohydroxyapatite for Y-TZP Implants

Miranda, R.B.P. ; Grenho, L. ; Carvalho, A. ; Fernandes, M.H. ; Monteiro, F.J. ; Cesar, P.F.

Journal of dental research, 2018-08, Vol.97 (9), p.1003-1009 [Periódico revisado por pares]

Los Angeles, CA: SAGE Publications

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Título:
Micropatterned Silica Films with Nanohydroxyapatite for Y-TZP Implants
Autor: Miranda, R.B.P. ; Grenho, L. ; Carvalho, A. ; Fernandes, M.H. ; Monteiro, F.J. ; Cesar, P.F.
Assuntos: Biological activity ; Calcium phosphates ; Cell adhesion & migration ; Cell culture ; Cell growth ; Cell proliferation ; Cell Proliferation - drug effects ; Ceramics ; Crack propagation ; Cytology ; Dental implants ; Dental Materials - chemistry ; Dentistry ; Flexural Strength ; Fractures ; Materials Testing ; Mechanical properties ; Micrography ; Microscopy, Electron, Scanning ; Morphology ; Nanostructures - chemistry ; Osteoblasts - drug effects ; Reproducibility of Results ; Scanning electron microscopy ; Silica ; Silicon Dioxide - chemistry ; Spectrometry, X-Ray Emission ; Statistical analysis ; Success ; Surface Properties ; Thin films ; Titanium ; Topography ; Transplants & implants ; X-ray spectroscopy ; Yttrium - chemistry ; Zirconia ; Zirconium - chemistry
É parte de: Journal of dental research, 2018-08, Vol.97 (9), p.1003-1009
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: This investigation aimed at developing micropatterned silica thin films (MSTFs) containing nanohydroxyapatite (nano-HA) microaggregates that were not completely covered by silica so that they could directly interact with the surrounding cells. The objectives were 1) to evaluate the effect of the presence of 2 films (MSTF with or without nano-HA addition) on the characteristic strength (σ0) and Weibull modulus (m) of a yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) and 2) to evaluate the effect of these 2 films, as applied onto the Y-TZP surface, on the morphology, orientation, and proliferation of MG63 cells. Sol-gel process and soft lithography were used to apply the MSTF onto the Y-TZP specimens. Three experimental groups were produced: Y-TZP, Y-TZP + MSTF, and Y-TZP + MSTF + sprayed nano-HA. All surfaces were characterized by scanning electron microscopy and energy-dispersive X-ray spectroscopy and tested for 4-point flexural strength (n = 30) in water at 37 °C. Weibull analysis was used to determine m and σ0 (maximum likelihood method). In vitro biological behavior was performed with human osteoblast-like cells (MG63). Y-TZP was successfully coated with MSFT and MSFT + nano-HA. Scanning electron microscopy micrographs indicated that the microaggregates of nano-HA were not entirely covered by the silica. There was no statistically significant difference among the experimental groups for σ0 and m. In the groups containing the films, the cells were elongated and aligned along the lines. The MSFT + nano-HA group showed significantly higher cell metabolic activity than that obtained for the Y-TZP group at day 7. This investigation was successful in producing an MSTF containing nano-HA microaggregates that remained exposed to the environment. The developed films did not jeopardize the structural reliability of a commercial Y-TZP, as confirmed by the Weibull statistics. The MG63 cells seeded over the films became elongated and aligned along the films’ micropatterned lines. Y-TZP specimens coated with MSTF and nano-HA showed a higher cell metabolic activity and proliferation after 7 d of culture when compared with uncoated Y-TZP.
Editor: Los Angeles, CA: SAGE Publications
Idioma: Inglês

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Micropatterned Silica Films with Nanohydroxyapatite for Y-TZP Implants

Miranda, R.B.P. ; Grenho, L. ; Carvalho, A. ; Fernandes, M.H. ; Monteiro, F.J. ; Cesar, P.F.

Los Angeles, CA: SAGE Publications

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