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Nanoleakage Distribution at Adhesive-Dentin Interfaces in 3D

Coutinho, E. ; Cardoso, M.V. ; Fernandes, C.P. ; Neves, A.A. ; Gouvea, C.V.D. ; Van Landuyt, K.L. ; De Munck, J. ; Van Meerbeek, B.

Journal of dental research, 2011-08, Vol.90 (8), p.1019-1025 [Periódico revisado por pares]

Los Angeles, CA: SAGE Publications

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Título:
Nanoleakage Distribution at Adhesive-Dentin Interfaces in 3D
Autor: Coutinho, E. ; Cardoso, M.V. ; Fernandes, C.P. ; Neves, A.A. ; Gouvea, C.V.D. ; Van Landuyt, K.L. ; De Munck, J. ; Van Meerbeek, B.
Assuntos: Adhesives ; Biological and medical sciences ; Biomaterials ; Coloring Agents ; Dental Bonding - adverse effects ; Dental Leakage - diagnosis ; Dental Leakage - etiology ; Dentin ; Dentin - pathology ; Dentin-Bonding Agents ; Dentistry ; Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics ; Imaging, Three-Dimensional - methods ; Interfaces ; Maxillofacial surgery. Dental surgery. Orthodontics ; Medical sciences ; Microscopy, Electron, Scanning - methods ; Monte Carlo Method ; Nitrates ; Physical characteristics ; Resin Cements ; Scattering, Radiation ; Silver ; Silver Nitrate ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Tomography ; Tomography - methods
É parte de: Journal of dental research, 2011-08, Vol.90 (8), p.1019-1025
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SourceType-Scholarly Journals-1
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Descrição: In spite of its role in the degradation of tooth-biomaterial interfaces, reports on nanoleakage are largely inconsistent. The aim of this work was to assess nanoleakage patterns qualitatively and quantitatively in 3D, to determine the influence of direction, position, and inclination of the field-of-view. Therefore, we applied a gold-standard 3-step etch-and-rinse adhesive to bur-cut dentin surfaces, after which interface samples were sectioned, infiltrated with an ammoniacal silver-nitrate solution, and embedded by common TEM procedures. High-resolution 3D models of interfaces were then generated by FIB and electron tomography, following strict conditions determined by Monte Carlo simulations. Inverted images in FIB tomography disclosed morphological characteristics analogous to those revealed by TEM. Quantitative analysis revealed large variations in silver-nitrate uptake between 2D image projections in different directions. Furthermore, silver-nitrate fractions in individual 2D image projections were seldom related to the total 3D volumetric fraction. Electron tomography showed that inclination also affected the morphology of silver-nitrate patterns. In conclusion, conventional nanoleakage evaluation is heavily influenced by direction, position, and inclination of the field-of-view, and thus may contain artifacts.
Editor: Los Angeles, CA: SAGE Publications
Idioma: Inglês

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Nanoleakage Distribution at Adhesive-Dentin Interfaces in 3D

Coutinho, E. ; Cardoso, M.V. ; Fernandes, C.P. ; Neves, A.A. ; Gouvea, C.V.D. ; Van Landuyt, K.L. ; De Munck, J. ; Van Meerbeek, B.

Los Angeles, CA: SAGE Publications

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