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Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced by Lactobacillus sp. A-2 metabolites

Zhang, Guoliang; Zhang, Jie; Wang, Xinyu; Yang, Wenqin; Sun, Zhihui; Kumar, Chaurasia Nitesh; Guan, Hong; Guan, Jian

Journal of Applied Oral Science; v. 22 n. 4 (2014); 282-286

Universidade de São Paulo. Faculdade de Odontologia de Bauru 2014-07-01

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  • Título:
    Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced by Lactobacillus sp. A-2 metabolites
  • Autor: Zhang, Guoliang; Zhang, Jie; Wang, Xinyu; Yang, Wenqin; Sun, Zhihui; Kumar, Chaurasia Nitesh; Guan, Hong; Guan, Jian
  • É parte de: Journal of Applied Oral Science; v. 22 n. 4 (2014); 282-286
  • Descrição: Objective: To study the effect of Lactobacillus sp. A-2 metabolites on viability of CAL-27 cells and apoptosis in CAL-27 cells. Methods: Lactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2) were obtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium and whey-inulin medium; the cultured CAL-27 cells were treated with different concentrations of LM1 and LM2 (0, 3, 6, 12, 24, 48 mg/mL) and assayed by methyl thiazolyltetrazolium (MTT) method; morphological changes of apoptotic cell were observed under fluorescence microscopy by acridine orange (Ao) fluorescent staining; flow cytometry method (FCM) and agarose gel electrophoresis were used to detect the apoptosis of CAL-27 cells treated LM1 and LM2. Results: The different concentrations of LM1 and LM2 could restrain the growth of CAL-27 cells, and in a dose-dependent manner; the apoptosis of CAL-27 cells was obviously induced and was time-dependent. Conclusions: Viability of CAL-27 cells was inhibited by Lactobacillus sp. A-2 metabolites; Lactobacillus sp. A-2 metabolites could induce CAL-27 cells apoptosis; study on the bioactive compounds in the Lactobacillus sp. A-2 metabolites and their molecular mechanism is in progress.
  • Títulos relacionados: https://www.revistas.usp.br/jaos/article/view/84014/86861
  • Editor: Universidade de São Paulo. Faculdade de Odontologia de Bauru
  • Data de criação/publicação: 2014-07-01
  • Formato: Adobe PDF
  • Idioma: Inglês
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