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The DNA Glycosylase T:G Mismatch-specific Thymine DNA Glycosylase Represses Thyroid Transcription Factor-1-activated Transcription

Missero, Caterina ; Pirro, Maria Teresa ; Simeone, Silvana ; Pischetola, Michele ; Di Lauro, Roberto

The Journal of biological chemistry, 2001-09, Vol.276 (36), p.33569-33575 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
The DNA Glycosylase T:G Mismatch-specific Thymine DNA Glycosylase Represses Thyroid Transcription Factor-1-activated Transcription
Autor: Missero, Caterina ; Pirro, Maria Teresa ; Simeone, Silvana ; Pischetola, Michele ; Di Lauro, Roberto
Assuntos: Ajuba protein ; Animals ; Cell Line ; COS Cells ; DNA, Complementary - metabolism ; Dose-Response Relationship, Drug ; Fungal Proteins - metabolism ; HeLa Cells ; HIPK2 protein ; Humans ; Molecular Sequence Data ; Mutation ; N-Glycosyl Hydrolases - chemistry ; Nuclear Proteins - metabolism ; Phosphorylation ; Precipitin Tests ; Protein Binding ; Protein Serine-Threonine Kinases - metabolism ; Protein Structure, Tertiary ; Rats ; Thymine DNA Glycosylase ; Thyroid Gland - metabolism ; Thyroid Nuclear Factor 1 ; thyroid transcription factor 1 ; Transcription Factors - metabolism ; Transcription, Genetic ; Transcriptional Activation ; Transfection ; TTF-1 protein ; Two-Hybrid System Techniques
É parte de: The Journal of biological chemistry, 2001-09, Vol.276 (36), p.33569-33575
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SourceType-Scholarly Journals-1
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Descrição: The transcription factor thyroid transcription factor-1 (TTF-1) is a homeodomain-containing protein that belongs to the NK2 family of genes involved in organogenesis. TTF-1 is required for normal development of the forebrain, lung, and thyroid. In a search for factors that regulate TTF-1 transcriptional activity, we isolated three genes (T:G mismatch-specific thymine DNA glycosylase (TDG), homeodomain-interacting protein kinase 2 (HIPK2), and Ajuba), whose products can interact with TTF-1 in yeast and in mammalian cells. TDG is an enzyme involved in base excision repair. In the present paper, we show that TDG acts as a strong repressor of TTF-1 transcriptional activity in a dose-dependent manner, while HIPK2 and Ajuba display no effect on TTF-1 activity, at least under the tested conditions. TDG-mediated inhibition occurs specifically on TTF-1-responsive promoters in thyroid and non thyroid cells. TDG associates with TTF-1 in mammalian cells through the TTF-1 carboxyl-terminal activation domain and is independent of the homeodomain. These findings reveal a previously unsuspected role for the repair enzyme TDG as a transcriptional repressor and open new routes toward the understanding of the regulation of TTF-1 transcriptional activity.
Editor: United States: Elsevier Inc
Idioma: Inglês

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The DNA Glycosylase T:G Mismatch-specific Thymine DNA Glycosylase Represses Thyroid Transcription Factor-1-activated Transcription

Missero, Caterina ; Pirro, Maria Teresa ; Simeone, Silvana ; Pischetola, Michele ; Di Lauro, Roberto

United States: Elsevier Inc

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