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Focus assay on RD114 virus QN10S cells

Sakaguchi, S.(Kyoto Univ. (Japan)) ; Baba, K ; Ishikawa, M ; Yoshikawa, R ; Shojima, T ; Miyazawa, T

Journal of Veterinary Medical Science, 2008, Vol.70(12), pp.1383-1386 [Periódico revisado por pares]

Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE

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Título:
Focus assay on RD114 virus QN10S cells
Autor: Sakaguchi, S.(Kyoto Univ. (Japan)) ; Baba, K ; Ishikawa, M ; Yoshikawa, R ; Shojima, T ; Miyazawa, T
Assuntos: Animals ; Biological Assay - veterinary ; CATS ; Cats - virology ; CELL CULTURE ; Cell Line, Tumor ; CHAT ; CULTIVO DE CELULAS ; CULTURE DE CELLULE ; endogenous retrovirus ; Fibroblasts - cytology ; Fibroblasts - virology ; focus assay ; GATO ; MONITORING ; RD114 ; RETROVIRIDAE ; Retroviridae - classification ; SURVEILLANCE ; VIGILANCIA ; VIROLOGIA ; VIROLOGIE ; VIROLOGY ; Virus Cultivation - veterinary
É parte de: Journal of Veterinary Medical Science, 2008, Vol.70(12), pp.1383-1386
Notas: 2009002157
L73
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Cats harbor an infectious endogenous retrovirus, named RD114 virus. It is therefore necessary to monitor RD114 virus production in feline cells which are used for biological products as substrates. In this study, a feline sarcoma-positive leukemia-negative (S+L-) fibroblast cell line, named QN10S cells, was found to be highly susceptible to RD114 pseudotype viruses. The cells were transformed by infection with RD114 virus and the numbers of foci could be counted. The sensitivity of the focus assay was lower than that of the LacZ marker rescue assay in detecting RD114 virus. Although the assay is not suitable to detect a small amount of the virus, the assay will be useful for virological studies of RD114 virus.
Editor: Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE
Idioma: Inglês

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Focus assay on RD114 virus QN10S cells

Sakaguchi, S.(Kyoto Univ. (Japan)) ; Baba, K ; Ishikawa, M ; Yoshikawa, R ; Shojima, T ; Miyazawa, T

Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE

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