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HIV Type 1 pol Gene Diversity and Antiretroviral Drug Resistance Mutations in Santos, Brazil

DE SA-FILHO, Dercy José ; DA SILVA SOARES, Marta ; CANDIDO, Valéria ; HENRIQUE GAGLIANI, Luiz ; CAVALIERE, Elizabeth ; SOBHIE DIAZ, Ricardo ; MONTANI CASEIRO, Marcos

AIDS research and human retroviruses, 2008-03, Vol.24 (3), p.347-353 [Periódico revisado por pares]

Larchmont, NY: Liebert

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Título:
HIV Type 1 pol Gene Diversity and Antiretroviral Drug Resistance Mutations in Santos, Brazil
Autor: DE SA-FILHO, Dercy José ; DA SILVA SOARES, Marta ; CANDIDO, Valéria ; HENRIQUE GAGLIANI, Luiz ; CAVALIERE, Elizabeth ; SOBHIE DIAZ, Ricardo ; MONTANI CASEIRO, Marcos
Assuntos: Adult ; AIDS/HIV ; Amino Acid Sequence ; Amino Acid Substitution - genetics ; Anti-HIV Agents - pharmacology ; Biological and medical sciences ; Brazil ; Cluster Analysis ; Complications and side effects ; Drug resistance ; Drug Resistance, Viral - genetics ; Drug therapy ; Female ; Fundamental and applied biological sciences. Psychology ; Gene mutations ; Genotype ; HIV infection ; HIV Infections - virology ; HIV Protease - genetics ; HIV Reverse Transcriptase - genetics ; HIV-1 - drug effects ; HIV-1 - genetics ; HIV-1 - isolation & purification ; Human immunodeficiency virus 1 ; Human viral diseases ; Humans ; Infectious diseases ; Male ; Medical sciences ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; Mutation ; Phylogeny ; pol Gene Products, Human Immunodeficiency Virus - chemistry ; pol Gene Products, Human Immunodeficiency Virus - genetics ; Polymorphism, Genetic ; Recombination, Genetic ; Retrovirus ; Reverse Transcriptase Polymerase Chain Reaction ; Risk factors ; RNA, Viral - blood ; RNA, Viral - genetics ; RNA, Viral - isolation & purification ; Selection, Genetic ; Sequence Alignment ; Sequence Analysis, DNA ; Viral diseases ; Virology
É parte de: AIDS research and human retroviruses, 2008-03, Vol.24 (3), p.347-353
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: HIV-1 antiretroviral drug resistance mutations in subtype B, F, and recombinants B/F in Santos, Brazil were characterized. We studied 83 samples from individuals enrolled at the Brazilian HIV/AIDS programs from Santos. These patients have been treated with multiantiretroviral therapy. Samples were collected in 2006; RNA was extracted from plasma and used as a target to amplify the pol gene of HIV-1. PCR products were sequenced on both strands, phylogenetic analyses were performed by neighbor-joining, and recombination was evaluated by bootscan. pol gene sequencing of the samples revealed that 54 strains belonged to subtype B, 4 were subtype F, 1 was subtype C, and 24 were B/F recombinants. Recombinant break points in 20 samples are the same identified in CRF28_BF and CRF29_BF. Drug resistance mutations identified in common to subtypes B, F, and recombinants B/F were protease inhibitors M46I/L (29%), I54V (24%), A71V (22%), and V82A/F (31%); reverse transcriptase nucleoside resistance mutations M41L (52%), D67N (30%), K70R (26%), M184V (88%), L210W (29%), T215Y/I/F (65%), and K219Q/E/N (28%); and reverse transcriptase nonnucleoside resistance mutation K103N (52%). Our results suggest that, in general, the same amino acids are emerging in both subtypes B, F, and recombinant forms BF due to the selective pressure of antiretrovirals. Recombinant break points in samples are the same as identified in CRF28_BF and CRF29_BF and are recognized as important for the evolution of the local epidemic.
Editor: Larchmont, NY: Liebert
Idioma: Inglês

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HIV Type 1 pol Gene Diversity and Antiretroviral Drug Resistance Mutations in Santos, Brazil

DE SA-FILHO, Dercy José ; DA SILVA SOARES, Marta ; CANDIDO, Valéria ; HENRIQUE GAGLIANI, Luiz ; CAVALIERE, Elizabeth ; SOBHIE DIAZ, Ricardo ; MONTANI CASEIRO, Marcos

Larchmont, NY: Liebert

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