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PD-1 identifies the patient-specific CD8⁺ tumor-reactive repertoire infiltrating human tumors

Gros, Alena ; Robbins, Paul F ; Yao, Xin ; Li, Yong F ; Turcotte, Simon ; Tran, Eric ; Wunderlich, John R ; Mixon, Arnold ; Farid, Shawn ; Dudley, Mark E ; Hanada, Ken-Ichi ; Almeida, Jorge R ; Darko, Sam ; Douek, Daniel C ; Yang, James C ; Rosenberg, Steven A

The Journal of clinical investigation, 2014-05, Vol.124 (5), p.2246-2259 [Periódico revisado por pares]

United States: American Society for Clinical Investigation

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Título:
PD-1 identifies the patient-specific CD8⁺ tumor-reactive repertoire infiltrating human tumors
Autor: Gros, Alena ; Robbins, Paul F ; Yao, Xin ; Li, Yong F ; Turcotte, Simon ; Tran, Eric ; Wunderlich, John R ; Mixon, Arnold ; Farid, Shawn ; Dudley, Mark E ; Hanada, Ken-Ichi ; Almeida, Jorge R ; Darko, Sam ; Douek, Daniel C ; Yang, James C ; Rosenberg, Steven A
Assuntos: Adoptive Transfer ; Antigens, CD - genetics ; Antigens, CD - immunology ; CD8-Positive T-Lymphocytes - immunology ; CD8-Positive T-Lymphocytes - pathology ; Cell Line, Tumor ; Female ; Hepatitis A Virus Cellular Receptor 2 ; Humans ; Male ; Melanoma - genetics ; Melanoma - immunology ; Melanoma - pathology ; Melanoma - therapy ; Membrane Proteins - genetics ; Membrane Proteins - immunology ; Programmed Cell Death 1 Receptor - genetics ; Programmed Cell Death 1 Receptor - immunology ; Receptors, Antigen, T-Cell, alpha-beta - genetics ; Receptors, Antigen, T-Cell, alpha-beta - immunology ; Tumor Microenvironment - genetics ; Tumor Microenvironment - immunology ; Tumor Necrosis Factor Receptor Superfamily, Member 9 - genetics ; Tumor Necrosis Factor Receptor Superfamily, Member 9 - immunology
É parte de: The Journal of clinical investigation, 2014-05, Vol.124 (5), p.2246-2259
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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Descrição: Adoptive transfer of tumor-infiltrating lymphocytes (TILs) can mediate regression of metastatic melanoma; however, TILs are a heterogeneous population, and there are no effective markers to specifically identify and select the repertoire of tumor-reactive and mutation-specific CD8⁺ lymphocytes. The lack of biomarkers limits the ability to study these cells and develop strategies to enhance clinical efficacy and extend this therapy to other malignancies. Here, we evaluated unique phenotypic traits of CD8⁺ TILs and TCR β chain (TCRβ) clonotypic frequency in melanoma tumors to identify patient-specific repertoires of tumor-reactive CD8⁺ lymphocytes. In all 6 tumors studied, expression of the inhibitory receptors programmed cell death 1 (PD-1; also known as CD279), lymphocyte-activation gene 3 (LAG-3; also known as CD223), and T cell immunoglobulin and mucin domain 3 (TIM-3) on CD8⁺ TILs identified the autologous tumor-reactive repertoire, including mutated neoantigen-specific CD8⁺ lymphocytes, whereas only a fraction of the tumor-reactive population expressed the costimulatory receptor 4-1BB (also known as CD137). TCRβ deep sequencing revealed oligoclonal expansion of specific TCRβ clonotypes in CD8⁺PD-1⁺ compared with CD8⁺PD-1- TIL populations. Furthermore, the most highly expanded TCRβ clonotypes in the CD8⁺ and the CD8⁺PD-1⁺ populations recognized the autologous tumor and included clonotypes targeting mutated antigens. Thus, in addition to the well-documented negative regulatory role of PD-1 in T cells, our findings demonstrate that PD-1 expression on CD8⁺ TILs also accurately identifies the repertoire of clonally expanded tumor-reactive cells and reveal a dual importance of PD-1 expression in the tumor microenvironment.
Editor: United States: American Society for Clinical Investigation
Idioma: Inglês

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PD-1 identifies the patient-specific CD8⁺ tumor-reactive repertoire infiltrating human tumors

Gros, Alena ; Robbins, Paul F ; Yao, Xin ; Li, Yong F ; Turcotte, Simon ; Tran, Eric ; Wunderlich, John R ; Mixon, Arnold ; Farid, Shawn ; Dudley, Mark E ; Hanada, Ken-Ichi ; Almeida, Jorge R ; Darko, Sam ; Douek, Daniel C ; Yang, James C ; Rosenberg, Steven A

United States: American Society for Clinical Investigation

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