Idioma:

Mite serine protease activates protease-activated receptor-2 and induces cytokine release in human keratinocytes

Kato, T ; Takai, T ; Fujimura, T ; Matsuoka, H ; Ogawa, T ; Murayama, K ; Ishii, A ; Ikeda, S ; Okumura, K ; Ogawa, H

Allergy (Copenhagen), 2009-09, Vol.64 (9), p.1366-1374 [Periódico revisado por pares]

Oxford, UK: Oxford, UK : Blackwell Publishing Ltd

Texto completo disponível

Citações Citado por

Enviar para

Título:
Mite serine protease activates protease-activated receptor-2 and induces cytokine release in human keratinocytes
Autor: Kato, T ; Takai, T ; Fujimura, T ; Matsuoka, H ; Ogawa, T ; Murayama, K ; Ishii, A ; Ikeda, S ; Okumura, K ; Ogawa, H
Assuntos: Allergic diseases ; Allergies ; Animals ; Antigens, Dermatophagoides - metabolism ; Antigens, Dermatophagoides - pharmacology ; Arthropod Proteins ; atopic dermatitis ; Biological and medical sciences ; Calcium - metabolism ; Cells, Cultured ; Cysteine Endopeptidases ; cysteine protease ; Cytokines ; Dermatitis ; Dermatitis, Atopic - immunology ; Dermatology ; Dermatophagoides pteronyssinus ; Dust ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Granulocyte-Macrophage Colony-Stimulating Factor - metabolism ; house dust mite ; Humans ; Immunopathology ; Interleukin-8 - metabolism ; keratinocyte ; Keratinocytes - drug effects ; Keratinocytes - immunology ; Medical sciences ; Peptides - pharmacology ; proinflammatory cytokine response ; protease-activated receptor-2 ; Proteases ; Pyroglyphidae - enzymology ; Pyroglyphidae - immunology ; Receptor, PAR-2 - agonists ; Receptor, PAR-2 - metabolism ; Recombinant Proteins - metabolism ; Recombinant Proteins - pharmacology ; RNA, Messenger - metabolism ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis ; serine protease ; Serine Proteases - immunology ; Serine Proteases - pharmacology ; Skin allergic diseases. Stinging insect allergies
É parte de: Allergy (Copenhagen), 2009-09, Vol.64 (9), p.1366-1374
Notas: http://dx.doi.org/10.1111/j.1398-9995.2009.02023.x
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
Descrição: House dust mites produce serine and cysteine proteases. Mite-derived proteases have been suggested to be involved in the pathogenesis of allergies; however, whether mite-derived serine protease activity can stimulate keratinocytes remains unknown. We examined the activation of primary human keratinocytes by serine protease-rich extract of whole mite culture and compared with that by recombinant group 1 allergens (rDer f 1 and rDer p 1), which exclusively exhibit cysteine protease activity. Protease activity of whole mite culture extract (WCE), rDer f 1 and rDer p 1 induced the release of IL-8 and granulocyte-macrophage colony-stimulating factor. Protease activity of WCEs induced a significant upregulation of their mRNA expression but rDer f 1 had much less effect. Protease activity of the WCE stimulated intracellular Ca²⁺ mobilization but rDer f 1 and rDer p 1 did not. The mobilization induced by agonists for the human protease-activated receptor (PAR)-2, an agonist peptide or trypsin, was diminished by pre-incubation of keratinocytes with WCE. rDer f 1 inefficiently cleaved a synthetic N-terminal peptide of PAR-2 at different sites from trypsin, but the resultant peptides did not stimulate the release of interleukin-8. The results suggest that mite-derived serine protease activity may contribute to the pathogenesis of atopic dermatitis by activating keratinocytes via PAR-2 activation but cysteine protease activity of Der f 1 and Der p 1 acts via another mechanism.
Editor: Oxford, UK: Oxford, UK : Blackwell Publishing Ltd
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Mite serine protease activates protease-activated receptor-2 and induces cytokine release in human keratinocytes

Kato, T ; Takai, T ; Fujimura, T ; Matsuoka, H ; Ogawa, T ; Murayama, K ; Ishii, A ; Ikeda, S ; Okumura, K ; Ogawa, H

Oxford, UK: Oxford, UK : Blackwell Publishing Ltd

Buscando em bases de dados remotas. Favor aguardar.