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Phosphoenolpyruvate carboxylase (PEPC) and PEPC-kinase (PEPC-k) isoenzymes in Arabidopsis thaliana: role in control and abiotic stress conditions

Feria, Ana B. ; Bosch, Nadja ; Sánchez, Alfonso ; Nieto-Ingelmo, Ana I. ; de la Osa, Clara ; Echevarría, Cristina ; García-Mauriño, Sofía ; Monreal, Jose Antonio

Planta, 2016-10, Vol.244 (4), p.901-913 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Science + Business Media

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Título:
Phosphoenolpyruvate carboxylase (PEPC) and PEPC-kinase (PEPC-k) isoenzymes in Arabidopsis thaliana: role in control and abiotic stress conditions
Autor: Feria, Ana B. ; Bosch, Nadja ; Sánchez, Alfonso ; Nieto-Ingelmo, Ana I. ; de la Osa, Clara ; Echevarría, Cristina ; García-Mauriño, Sofía ; Monreal, Jose Antonio
Assuntos: Agriculture ; Arabidopsis ; Arabidopsis - enzymology ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Biomedical and Life Sciences ; Blotting, Western ; Ecology ; Forestry ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Plant ; Isoenzymes - genetics ; Isoenzymes - metabolism ; Life Sciences ; Mutation ; ORIGINAL ARTICLE ; Phosphates - metabolism ; Phosphoenolpyruvate Carboxylase - genetics ; Phosphoenolpyruvate Carboxylase - metabolism ; Plant Roots - enzymology ; Plant Roots - genetics ; Plant Roots - growth & development ; Plant Sciences ; Plant Shoots - enzymology ; Plant Shoots - genetics ; Plant Shoots - growth & development ; Protein-Serine-Threonine Kinases - genetics ; Protein-Serine-Threonine Kinases - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Salinity ; Stress, Physiological
É parte de: Planta, 2016-10, Vol.244 (4), p.901-913
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Phosphoenolpyruvate carboxylase (PEPC) and its dedicated kinase (PEPC-k) are ubiquitous plant proteins implicated in many physiological processes. This work investigates specific roles for the three plant-type PEPC (PTPC) and the two PEPC-k isoenzymes in Arabidopsis thaliana. The lack of any of the PEPC isoenzymes reduced growth parameters under optimal growth conditions. PEPC activity was decreased in shoots and roots of ppc2 and ppc3 mutants, respectively. Phosphate starvation increased the expression of all PTPC and PPCK genes in shoots, but only PPC3 and PPCK2 in roots. The absence of any of these two proteins was not compensated by other isoforms in roots. The effect of salt stress on PTPC and PPCK expression was modest in shoots, but PPC3 was markedly increased in roots. Interestingly, both stresses decreased root growth in each of the mutants except for ppc3. This mutant had a stressed phenotype in control conditions (reduced root growth and high level of stress molecular markers), but was unaffected in their response to high salinity. Salt stress increased PEPC activity, its phosphorylation state, and L-malate content in roots, all these responses were abolished in the ppc3 mutant. Our results highlight the importance of the PPC3 isoenzyme for the normal development of plants and for root responses to stress.
Editor: Berlin/Heidelberg: Springer Science + Business Media
Idioma: Inglês

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Phosphoenolpyruvate carboxylase (PEPC) and PEPC-kinase (PEPC-k) isoenzymes in Arabidopsis thaliana: role in control and abiotic stress conditions

Feria, Ana B. ; Bosch, Nadja ; Sánchez, Alfonso ; Nieto-Ingelmo, Ana I. ; de la Osa, Clara ; Echevarría, Cristina ; García-Mauriño, Sofía ; Monreal, Jose Antonio

Berlin/Heidelberg: Springer Science + Business Media

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