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Skyline Performs as Well as Vendor Software in the Quantitative Analysis of Serum 25-Hydroxy Vitamin D and Vitamin D Binding Globulin

Henderson, Clark M ; Shulman, Nicholas J ; MacLean, Brendan ; MacCoss, Michael J ; Hoofnagle, Andrew N

Clinical chemistry (Baltimore, Md.), 2018-02, Vol.64 (2), p.408-410 [Periódico revisado por pares]

United States: American Association for Clinical Chemistry, Inc

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  • Título:
    Skyline Performs as Well as Vendor Software in the Quantitative Analysis of Serum 25-Hydroxy Vitamin D and Vitamin D Binding Globulin
  • Autor: Henderson, Clark M ; Shulman, Nicholas J ; MacLean, Brendan ; MacCoss, Michael J ; Hoofnagle, Andrew N
  • Assuntos: Calibration ; Chromatography ; Data analysis ; Experiments ; Globulins ; Laboratories ; Mass spectrometry ; Peptides ; Proteins ; Proteomics ; Quantitative analysis ; Scientific imaging ; Software packages ; Vitamin D
  • É parte de: Clinical chemistry (Baltimore, Md.), 2018-02, Vol.64 (2), p.408-410
  • Notas: SourceType-Other Sources-1
    content type line 63
    ObjectType-Correspondence-1
  • Descrição: Because the chromatographic characteristics are slightly different for peptides and small molecules, we quantitatively analyzed serum concentrations of 25-hydroxy vitamin D [25(OH)D]1 and vitamin D binding globulin (VDBG), as previously described (2, 3). Briefly, 25(OH)D was immunoaffinity enriched from 154 single-donor human serum samples, and the derivatized extracted analytes and stable isotope-labeled analogs were chromatographically separated using Waters Acquity UPLC coupled online via an electrospray ionization source operating in positive ion detection mode for analysis by multiple reaction monitoring on a Waters Xevo triple quadrupole mass spectrometer. For VDBG analysis, 164 singledonor human serum samples were tryptically digested with isotopelabeled peptides spiked before digestion, and peptides were analyzed using the same instrumentation as for 25(OH)D. Peak area data were analyzed, and VDBG concentrations were calculated based on a 4-point calibration curve made from human serum diluted with chicken serum.
  • Editor: United States: American Association for Clinical Chemistry, Inc
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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