Idioma:

Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis

FUJIWARA, Ricardo Toshio ; VALE, André Macedo ; BETHONY, Jeffrey Michael ; FRANK, Glen ; NASCIMENTO, Evaldo ; GENARO, Odair ; MAYRINK, Wilson ; REED, Steven ; CAMPOS-NETO, Antonio ; FRANCA DA SILVA, Joao Carlos ; DA COSTA, Roberto Teodoro ; QUETZ, Josiane Da ; MARTINS FILHO, Olindo Assis ; REIS, Alexandre Barbosa ; CORREA OLIVEIRA, Rodrigo ; MACHADO-COELHO, George Lins ; BUENO, Lilian Lacerda

Veterinary research (Paris), 2005-09, Vol.36 (5-6), p.827-838 [Periódico revisado por pares]

Les Ulis: EDP Sciences

Texto completo disponível

Citações Citado por

Enviar para

Título:
Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis
Autor: FUJIWARA, Ricardo Toshio ; VALE, André Macedo ; BETHONY, Jeffrey Michael ; FRANK, Glen ; NASCIMENTO, Evaldo ; GENARO, Odair ; MAYRINK, Wilson ; REED, Steven ; CAMPOS-NETO, Antonio ; FRANCA DA SILVA, Joao Carlos ; DA COSTA, Roberto Teodoro ; QUETZ, Josiane Da ; MARTINS FILHO, Olindo Assis ; REIS, Alexandre Barbosa ; CORREA OLIVEIRA, Rodrigo ; MACHADO-COELHO, George Lins ; BUENO, Lilian Lacerda
Assuntos: Animal biology ; Animal genetics ; Animals ; Antibodies, Protozoan - biosynthesis ; Antigens, Protozoan - immunology ; Applied microbiology ; Biochemistry, Molecular Biology ; Biological and medical sciences ; Cell Behavior ; Cellular Biology ; Dog Diseases - immunology ; Dog Diseases - parasitology ; Dogs ; Fundamental and applied biological sciences. Psychology ; Genetics ; Human protozoal diseases ; Immunology ; Infectious diseases ; Leishmania ; Leishmaniasis, Visceral - immunology ; Leishmaniasis, Visceral - veterinary ; Leshmaniasis ; Life Sciences ; Medical sciences ; Microbiology ; Microbiology and Parasitology ; Molecular biology ; Neurons and Cognition ; Parasitic diseases ; Protozoal diseases ; Protozoan Vaccines - immunology ; Santé publique et épidémiologie ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccines, Synthetic - immunology
É parte de: Veterinary research (Paris), 2005-09, Vol.36 (5-6), p.827-838
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Control of canine visceral leishmaniasis (VL) remains a difficult and serious problem mostly because there is no reliable and effective vaccine available to prevent this disease. A mixture of three recombinant leishmanial antigens (TSA, LeIF and LmSTI1) encoded by three genes highly conserved in the Leishmania genus have been shown to induce excellent protection against infection in both murine and simian models of cutaneous leishmaniasis. A human clinical trial with these antigens is currently underway. Because of the high degree of conservation, these antigens might be useful vaccine candidates for VL as well. In the present study, using the dog model of the visceral disease, we evaluated the immunogenicity of these three antigens formulated with two different adjuvants, MPL-SE and AdjuPrime. The results were compared with a whole parasite vaccine formulated with BCG as the adjuvant. In order to investigate if sensitization with the recombinant antigens would result in recognition of the corresponding native parasite antigens upon infection, the animals were exposed for four weeks after the termination of the immunization protocol with the recombinant antigens to a low number of L. chagasi promastigotes, an etiological agent of VL. Immune response was evaluated by quantitative ELISA in the animal sera before and after exposure to the viable parasites. Both antigen specific IgG1 and IgG2 antibody levels were measured. Immunization of dogs with the recombinant antigens formulated in either MPL-SE or AdjuPrime resulted in high antibody levels particularly to LmSTI1. In addition, this immunization although to low levels, resulted in the development of antibody response to the whole parasite lysate. Importantly, experimental exposure with low numbers of culture forms of L. chagasi promastigotes caused a clear boost in the immune response to both the recombinant antigens and the corresponding native molecules. The boost response was predominantly of the IgG2 isotype in animals primed with the recombinant antigens plus MPL-SE. In contrast, animals primed with the recombinant antigens formulated in AdjuPrime as well as animals vaccinated with crude antigen preparation responded with mixed IgG1/IgG2 isotypes. These results point to the possible use of this antigen cocktail formulated with the adjuvant MPL-SE in efficacy field trials against canine VL.
Editor: Les Ulis: EDP Sciences
Idioma: Inglês

Links

Voltar para lista de resultados

Anterior Resultado 8 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis

Les Ulis: EDP Sciences

Buscando em bases de dados remotas. Favor aguardar.