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Leishmania identification by PCR of Giemsa-stained lesion imprint slides stored for up to 36 years

Volpini, Â.C. ; Marques, M.J. ; Lopes dos Santos, S. ; Machado-Coelho, G.L. ; Mayrink, W. ; Romanha, A.J.

Clinical microbiology and infection, 2006-08, Vol.12 (8), p.815-818 [Periódico revisado por pares]

Oxford, UK: Elsevier Ltd

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Título:
Leishmania identification by PCR of Giemsa-stained lesion imprint slides stored for up to 36 years
Autor: Volpini, Â.C. ; Marques, M.J. ; Lopes dos Santos, S. ; Machado-Coelho, G.L. ; Mayrink, W. ; Romanha, A.J.
Assuntos: Animals ; Azure Stains ; Biological and medical sciences ; Diagnosis ; DNA, Protozoan - analysis ; Humans ; identification ; Infectious diseases ; kDNA ; Leishmania ; Leishmania braziliensis - isolation & purification ; Leishmaniasis, Cutaneous - diagnosis ; Medical sciences ; PCR ; Polymerase Chain Reaction - methods ; Polymorphism, Restriction Fragment Length ; species identification ; Specimen Handling ; Time Factors ; Viannia braziliensis
É parte de: Clinical microbiology and infection, 2006-08, Vol.12 (8), p.815-818
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: This study examined the ability of PCR to amplify Leishmania DNA, stored on Giemsa-stained slides, from American cutaneous leishmaniasis (ACL) patients. In total, 475 slides stored for up to 36 years were obtained from an outpatient clinic in a Brazilian ACL-endemic region, and Leishmania DNA was amplified from 395 (83.2%) of the DNA samples using primers specific for the minicircle kinetoplast DNA. Restriction fragment length polymorphism analysis of these amplicons demonstrated that Leishmania (Viannia) braziliensis was the only species present in these samples. The results demonstrated that archived Giemsa-stained slides can provide a Leishmania DNA source for performing clinical and epidemiological studies of leishmaniasis.
Editor: Oxford, UK: Elsevier Ltd
Idioma: Inglês

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Leishmania identification by PCR of Giemsa-stained lesion imprint slides stored for up to 36 years

Volpini, Â.C. ; Marques, M.J. ; Lopes dos Santos, S. ; Machado-Coelho, G.L. ; Mayrink, W. ; Romanha, A.J.

Oxford, UK: Elsevier Ltd

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