skip to main content
Idioma:
Primo Advanced Search
Primo Advanced Search Query Term
Primo Advanced Search prefilters
Busca Simples

The Fine Art of Destruction: A Guide to In‐Depth Glycoproteomic Analyses—Exploiting the Diagnostic Potential of Fragment Ions

Hoffmann, Marcus ; Pioch, Markus ; Pralow, Alexander ; Hennig, René ; Kottler, Robert ; Reichl, Udo ; Rapp, Erdmann

Proteomics (Weinheim), 2018-12, Vol.18 (24), p.e1800282-n/a [Periódico revisado por pares]

Germany

Texto completo disponível

Citações Citado por
  • Título:
    The Fine Art of Destruction: A Guide to In‐Depth Glycoproteomic Analyses—Exploiting the Diagnostic Potential of Fragment Ions
  • Autor: Hoffmann, Marcus ; Pioch, Markus ; Pralow, Alexander ; Hennig, René ; Kottler, Robert ; Reichl, Udo ; Rapp, Erdmann
  • Assuntos: fibrinogen ; glycoproteomics ; higher energy collision dissociation ; hydrophilic interaction liquid chromatography ; IgG ; lactotransferrin ; mass spectrometry ; N‐glycosylation ; oxonium ions ; O‐glycosylation ; proteinase K ; ribonuclease B
  • É parte de: Proteomics (Weinheim), 2018-12, Vol.18 (24), p.e1800282-n/a
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: The unambiguous mass spectrometric identification and characterization of glycopeptides is crucial to elucidate the micro‐ and macroheterogeneity of glycoproteins. Here, combining lower and stepped collisional energy fragmentation for the in‐depth and site‐specific analysis of N‐ and O‐glycopeptides is proposed. Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state‐of‐the‐art blueprint for conducting high‐quality in‐depth N‐ and O‐glycoproteomic analyses is provided. Further, a modified and improved version of cotton hydrophilic interaction liquid chromatography‐based solid phase extraction for glycopeptide enrichment is described. For the unambiguous identification of N‐glycopeptides, the use of a conserved yet, rarely employed‐fragmentation signature [Mpeptide+H+0,2X GlcNAc]+ is proposed. It is shown for the first time that this fragmentation signature can consistently be found across all N‐glycopeptides, but not on O‐glycopeptides. Moreover, the use of the relative abundance of oxonium ions to retrieve glycan structure information, for example, differentiation of hybrid‐ and high‐mannose‐type N‐glycans or differentiation between antenna GlcNAc and bisecting GlcNAc, is systematically and comprehensively evaluated. The findings may increase confidence and comprehensiveness in manual and software‐assisted glycoproteomics.
  • Editor: Germany
  • Idioma: Inglês
Links
Voltar para lista de resultados
Ir para página anteriorAnterior Resultado  8 AvançarIr para próxima página

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.