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13C−1H heteronuclear dipolar correlation studies of the hydrogen bonding of the quinones inRhodobacter sphaeroides R26 reaction centers

van Rossum, B. J. ; van Liemt, W. B. S. ; Gast, P. ; Lugtenburg, J. ; de Groot, H. J. M.

Applied magnetic resonance, 2007-03, Vol.31 (1-2), p.145-158 [Periódico revisado por pares]

Heidelberg: Springer Nature B.V

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  • Título:
    13C−1H heteronuclear dipolar correlation studies of the hydrogen bonding of the quinones inRhodobacter sphaeroides R26 reaction centers
  • Autor: van Rossum, B. J. ; van Liemt, W. B. S. ; Gast, P. ; Lugtenburg, J. ; de Groot, H. J. M.
  • Assuntos: Bonding strength ; Carbon 13 ; Correlation ; Ground state ; Hydrogen ; Hydrogen bonding ; Hydroxyl groups ; Labels ; NMR ; Nuclear magnetic resonance ; Photosynthesis ; Polarization ; Protons ; Quinones
  • É parte de: Applied magnetic resonance, 2007-03, Vol.31 (1-2), p.145-158
  • Descrição: The photosynthetic reaction center (RC) of the photosynthetic bacteriumRhodobacter sphaeroides R26 contains two quinones, QA and QB. Solid-state heteronuclear (1H−13C) dipolar correlation spectroscopy has been used to study the binding of the quinones in the ground state for RCs reconstituted with l-13C ubiquinone-10. Lee-Goldburg cross-polarization buildup curves are recorded to determine distancesrCH between the l-13C carbon labels and the protons involved in the polarization transfer. The l-13C of both QA and QB have intermolecular correlations with protons that resonate downfield, in the region of hydrogen-bonding protons. The distances between the carbon labels and the correlated protons are short, 0.21±0.01 nm. Hence the nuclear magnetic resonance provides evidence for strong hydrogen-bonding interactions at the l-C=O of both QA and QB for RCs in the ground state. The environment of the l-13C of the QB is structurally heterogeneous compared to that of the QA. The data can be reconciled with a strong H-bonding interaction of the l-C=O of QA with Ala M260 NH, and with complex hydrogen bonding involving NH of Ile-L224 and of Gly-L225, and possibly the Ser-L223 hydroxyl group of the l-C=O of the QB, in the proximal site.
  • Editor: Heidelberg: Springer Nature B.V
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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