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Therapeutic bioactive microcarriers: Co-delivery of growth factors and stem cells for bone tissue engineering

Perez, R.A. ; El-Fiqi, A. ; Park, J.-H. ; Kim, T.-H. ; Kim, J.-H. ; Kim, H.-W.

Acta biomaterialia, 2014-01, Vol.10 (1), p.520-530 [Periódico revisado por pares]

England: Elsevier Ltd

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Título:
Therapeutic bioactive microcarriers: Co-delivery of growth factors and stem cells for bone tissue engineering
Autor: Perez, R.A. ; El-Fiqi, A. ; Park, J.-H. ; Kim, T.-H. ; Kim, J.-H. ; Kim, H.-W.
Assuntos: Adsorption ; Animals ; Apatites - chemistry ; Bioactive glass ; Biocompatibility ; Biocompatible Materials - pharmacology ; Biomedical materials ; Bone and Bones - drug effects ; Bone and Bones - physiology ; Bone tissue engineering ; Bones ; Cell Line ; Cell Proliferation - drug effects ; Cytochromes c - metabolism ; Fibroblast Growth Factor 2 - pharmacology ; Glass ; Growth factor delivery ; Growth factors ; Humans ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - drug effects ; Mesenchymal Stromal Cells - metabolism ; Mice ; Microcarriers ; Microscopy, Electron, Scanning ; Microspheres ; Nitrogen - chemistry ; Particle Size ; Porosity ; Rats ; Stem Cell Transplantation ; Stem cells ; Stem Cells - cytology ; Stem Cells - drug effects ; Sustainability ; Sustainable release ; Tissue engineering ; Tissue Engineering - methods
É parte de: Acta biomaterialia, 2014-01, Vol.10 (1), p.520-530
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Mesoporous and bioactive silica-based microspheres demonstrated excellent biocompatibility to support cell growth and tissue responses as well as long-term delivery of growth factors, suggesting a novel therapeutic microcarrier system for bone tissue engineering. Novel microcarriers made of sol–gel-derived bioactive glasses were developed for delivering therapeutic molecules effectively while cultivating stem cells for bone tissue engineering. Silica sols with varying concentration of Ca (0–30mol.%) were formulated into microspheres ranging from 200 to 300μm under optimized conditions. A highly mesoporous structure was created, with mesopore sizes of 2.5–6.3nm and specific surface areas of 420–710m2g−1, which was highly dependent on the Ca concentration. Therapeutic molecules could be effectively loaded within the mesoporous microcarriers during microsphere formulation. Cytochrome C (cyt C), used as a model protein for the release study, was released in a highly sustainable manner, with an almost zero-order kinetics over a period of months; the amount released was ∼2% at 9days, and 15% at 40days. A slight increase in the release rate was observed in the microcarrier containing Ca, which was related to the dissolution rate and pore size. The presence of Ca accelerated the formation of hydroxyapatite on the surface of the microcarriers. Cells cultured on the bioactive microcarriers were well adhered and distributed, and proliferated actively, confirming the three-dimensional substrate role of the microcarriers. An in vivo study performed in a rat subcutaneous model demonstrated the satisfactory biocompatibility of the prepared microspheres. As a therapeutic target molecule, basic fibroblast growth factor (bFGF) was incorporated into the microcarriers. A slow release pattern similar to that of cyt C was observed for bFGF. Cells adhered and proliferated to significantly higher levels on the bFGF-loaded microcarriers, demonstrating the effective role of bFGF in cell proliferative potential. It is believed that the developed mesoporous bioactive glass microspheres represent a new class of therapeutic cell delivery carrier, potentially useful in the sustainable delivery of therapeutic molecules such as growth factors, as well as in the support of stem cell proliferation and osteogenesis for bone tissue engineering.
Editor: England: Elsevier Ltd
Idioma: Inglês

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Therapeutic bioactive microcarriers: Co-delivery of growth factors and stem cells for bone tissue engineering

Perez, R.A. ; El-Fiqi, A. ; Park, J.-H. ; Kim, T.-H. ; Kim, J.-H. ; Kim, H.-W.

England: Elsevier Ltd

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