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Dissociation and recombination between ligands and heme in a CO-sensing transcriptional activator CooA. A flash photolysis study

Kumazaki, S ; Nakajima, H ; Sakaguchi, T ; Nakagawa, E ; Shinohara, H ; Yoshihara, K ; Aono, S

The Journal of biological chemistry, 2000-12, Vol.275 (49), p.38378-38383 [Periódico revisado por pares]

United States

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Título:
Dissociation and recombination between ligands and heme in a CO-sensing transcriptional activator CooA. A flash photolysis study
Autor: Kumazaki, S ; Nakajima, H ; Sakaguchi, T ; Nakagawa, E ; Shinohara, H ; Yoshihara, K ; Aono, S
Assuntos: Bacterial Proteins - chemistry ; Bacterial Proteins - metabolism ; Carbon Monoxide ; CooA protein ; Heme - chemistry ; Heme - metabolism ; Hemeproteins - chemistry ; Hemeproteins - metabolism ; Kinetics ; Ligands ; Oxidation-Reduction ; Photolysis ; Rhodospirillum rubrum ; Rhodospirillum rubrum - metabolism ; Spectrophotometry ; Time Factors ; Trans-Activators - chemistry ; Trans-Activators - metabolism
É parte de: The Journal of biological chemistry, 2000-12, Vol.275 (49), p.38378-38383
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
Descrição: CooA from Rhodospirillum rubrum is a transcriptional activator in which a heme prosthetic group acts as a CO sensor and regulates the activity of the protein. In this study, the electronic relaxation of the heme, and the concurrent recombination between ligands and the heme at approximately 280 K were examined in an effort to understand the environment around the heme and the dynamics of the ligands. Upon photoexcitation of the reduced CooA at 400 nm, electronic relaxation of the heme occurred with time constants of 0.8 and 1.7 ps. The ligand rebinding was substantially completed with a time constant of 6.5 ps, followed by a slow relaxation process with a time constant of 173 ps. In the case of CO-bound CooA, relaxation of the excited heme occurred with two time constants, 1.1 and 2.4 ps, which were largely similar to those with reduced CooA. The subsequent CO recombination process was remarkably fast compared with that of other CO-bound heme proteins. It was well described as a biphasic geminate recombination process with time constants of 78 ps (60%) and 386 ps (30%). About 10% of the excited heme remained unligated at 1.9 ns. The dynamics of rebinding of CO thus will help us to understand how the physiologically relevant diatomic molecule approaches the heme binding site in CooA with picosecond resolution.
Editor: United States
Idioma: Inglês

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Dissociation and recombination between ligands and heme in a CO-sensing transcriptional activator CooA. A flash photolysis study

Kumazaki, S ; Nakajima, H ; Sakaguchi, T ; Nakagawa, E ; Shinohara, H ; Yoshihara, K ; Aono, S

United States

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