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Development of a polymerase chain reaction and a nonradioactive DNA probe for infectious laryngotracheitis virus

Abbas, F. (Veterinary Research Institute, Quetta, Balochistan, Pakistan.) ; Andreasen, J.R. Jr ; Jackwood, M.W

Avian diseases, 1996-01, Vol.40 (1), p.56-62 [Periódico revisado por pares]

United States: American Association of Avian Pathologists, Inc

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Título:
Development of a polymerase chain reaction and a nonradioactive DNA probe for infectious laryngotracheitis virus
Autor: Abbas, F. (Veterinary Research Institute, Quetta, Balochistan, Pakistan.) ; Andreasen, J.R. Jr ; Jackwood, M.W
Assuntos: ADN ; AMPLIFICATION CHAINE POLYMERASE ; Animals ; Base Sequence ; Chickens ; Chickens - virology ; DNA ; DNA Probes ; DNA, Viral - isolation & purification ; Herpesviridae ; Herpesviridae Infections - veterinary ; Herpesviridae Infections - virology ; Herpesvirus 1, Gallid - isolation & purification ; HIBRIDACION DE ADN ; HYBRIDATION D'ADN ; Molecular Sequence Data ; Mycoplasma gallisepticum ; Mycoplasma synoviae ; Polymerase chain reaction ; Polymerase Chain Reaction - veterinary ; Poultry Diseases - virology ; REACCION DE CADENAS DE POLIMERASA ; Sodium ; Vaccination ; VIRUS DE LA LARINGOTRAQUEITIS AVIAR ; VIRUS DE LA LTI ; Viruses
É parte de: Avian diseases, 1996-01, Vol.40 (1), p.56-62
Notas: 9614498
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Descrição: The polymerase chain reaction (PCR) was developed using infectious laryngotracheitis virus (ILTV) primers made from a portion of the ILTV thymidine kinase gene. DNA from various ILTV field isolates, from the USDA challenge strain of ILTV, and from commercial ILTV vaccines was specifically amplified. No amplification occurred using template DNA from uninfected chicken-embryo liver cells (CELC), several nonavian alphaherpesviruses, Mycoplasma gallisepticum, Mycoplasma synoviae, Pasteurella hemolytica, Escherichia coli, a group I avian adenovirus, fowl poxvirus, or a psittacid herpesvirus. The 647-base pair-amplified ILTV PCR product was labeled to create a nonradioactive, biotinylated DNA probe. Hybridization using the probe detected ILTV DNA. Both PCR and hybridization yielded positive results with ILTV DNA but not with the DNA of other pathogens. Hybridization was specific for ILTV using a stringent salt solution for a 30-min wash step or a somewhat less stringent salt solution for a 60-min wash step. However, slight hybridization occurred with CELC DNA when the less stringent salt solution was used in a 30-min wash step
Editor: United States: American Association of Avian Pathologists, Inc
Idioma: Inglês

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Development of a polymerase chain reaction and a nonradioactive DNA probe for infectious laryngotracheitis virus

Abbas, F. (Veterinary Research Institute, Quetta, Balochistan, Pakistan.) ; Andreasen, J.R. Jr ; Jackwood, M.W

United States: American Association of Avian Pathologists, Inc

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